Mosunov Andrei A, Pashkova Irina S, Sidorova Maria, Pronozin Artem, Lantushenko Anastasia O, Prylutskyy Yuriy I, Parkinson John A, Evstigneev Maxim P
Department of Physics, Sevastopol State University, Universitetskaya str., 33, 299053 Sevastopol, Crimea.
Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, Ukraine.
Phys Chem Chem Phys. 2017 Mar 1;19(9):6777-6784. doi: 10.1039/c6cp07140f.
We report a new analytical method that allows the determination of the magnitude of the equilibrium constant of complexation, K, of small molecules to C fullerene in aqueous solution. The developed method is based on the up-scaled model of C fullerene-ligand complexation and contains the full set of equations needed to fit titration datasets arising from different experimental methods (UV-Vis spectroscopy, H NMR spectroscopy, diffusion ordered NMR spectroscopy, DLS). The up-scaled model takes into consideration the specificity of C fullerene aggregation in aqueous solution and allows the highly dispersed nature of C fullerene cluster distribution to be accounted for. It also takes into consideration the complexity of fullerene-ligand dynamic equilibrium in solution, formed by various types of self- and hetero-complexes. These features make the suggested method superior to standard Langmuir-type analysis, the approach used to date for obtaining quantitative information on ligand binding with different nanoparticles.
我们报告了一种新的分析方法，该方法能够测定小分子与水溶 液中C富勒烯络合的平衡常数K的大小。所开发的方法基于C富勒烯 -配体络合的放大模型，包含拟合来自不同实验方法（紫外 -可见光谱、氢核磁共振光谱、扩散排序核磁共振光谱、动态光散射） 的滴定数据集所需的全套方程。放大模型考虑了C富勒烯在水溶液 中聚集的特异性，并能够解释C富勒烯簇分布的高度分散性质。它 还考虑了溶液中富勒烯 -配体动态平衡的复杂性，这种平衡由各种 类型的自络合物和杂络合物形成。这些特性使得所提出的方法优于 标准的朗缪尔型分析，即迄今为止用于获取配体与不同纳米颗粒 结合的定量信息的方法。
