Tan Meng-Xia, Xiong Wei, He Ling-Kun, He Ling-Kun, Yang Guo, Huang Li-Ping, Shen Yu-Lin, Liang Shang-Dong, Gao Yun
Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, China.
Department of Preventive Dentistry, The Affiliated Stomatological Hospital of Nanchang University, Nanchang 330019, China.
Sheng Li Xue Bao. 2017 Feb 25;69(1):89-95.
Trigeminal neuralgia (TN) is a kind of recurrent transient and severe pain that is limited to the trigeminal nerve in one or more branches. The clinical incidence of TN is high, which seriously affects the quality of life of the patients and is difficult to cure. The present study investigated the effects of tetramethylpyrazine (TMP) on TN induced by chronic constriction injury of the infraorbital nerve (ION-CCI) in rats. Adult male Sprague-Dawley rats were randomly assigned to four groups: sham, sham treated with TMP (Sham+TMP), TN model (TN), and TN treated with TMP (TN+TMP). The rat TN model was established by ION-CCI and TMP (50 mg/kg) was injected intraperitoneally once a day for 2 weeks after operation. The mechanical response threshold was tested by the electronic von Frey filaments. The expression of CGRP in the trigeminal ganglia (TGs) of rats on the operative side was detected by RT-PCR, immunohistochemical staining and Western blot. In 15 days after operation, TN group showed a robust decrease in mechanical response threshold as compared with sham group. From day 9 to day 15 after operation, TMP treatment significantly suppressed the TN-induced mechanical hyperalgesia (P < 0.05). On day 15 after operation, RT-PCR, immunohistochemical staining and Western blot analysis showed an obvious increase in expression level of CGRP in TGs of TN group compared with sham group, which was downregulated by TMP treatment (P < 0.05). These results suggested that TMP might have a therapeutic potential for the treatment of TN through regulating CGRP expression in the TGs.
三叉神经痛（TN）是一种反复发作的短暂性剧痛，局限于三叉神经的一个或多个分支。TN的临床发病率较高，严重影响患者的生活质量且难以治愈。本研究调查了川芎嗪（TMP）对大鼠眶下神经慢性压迫损伤（ION-CCI）诱导的TN的影响。成年雄性Sprague-Dawley大鼠被随机分为四组：假手术组、假手术+TMP处理组（Sham+TMP）、TN模型组（TN）和TN+TMP处理组。通过ION-CCI建立大鼠TN模型，术后每天腹腔注射TMP（50mg/kg），持续2周。用电子von Frey细丝测试机械反应阈值。通过RT-PCR、免疫组织化学染色和蛋白质印迹法检测手术侧大鼠三叉神经节（TGs）中降钙素基因相关肽（CGRP）的表达。术后15天，与假手术组相比，TN组机械反应阈值显著降低。术后第9天至第15天，TMP处理显著抑制了TN诱导的机械性痛觉过敏（P<0.05）。术后15天，RT-PCR、免疫组织化学染色和蛋白质印迹分析显示，与假手术组相比，TN组TGs中CGRP的表达水平明显升高，而TMP处理使其下调（P<0.05）。这些结果表明，TMP可能通过调节TGs中CGRP的表达对TN具有治疗潜力。
