Department of Biotechnology, Sri Krishnadevaraya University, Anantapur, India.
National Institute of Animal Biotechnology (NIAB), Hyderabad, India.
Sci Rep. 2017 Feb 20;7:42683. doi: 10.1038/srep42683.
Recent reports have demonstrated the role of phyto-constituents in modulating inflammatory responses. Mangiferin isolated from Mangifera indica is known to induce potent anti-oxidative, anti-diabetic and anti-inflammatory activity. However, the molecular mechanism of its anti-inflammatory activity is not properly understood. In this study we have isolated Mangiferin from the tubers of Pueraria tuberosa (PT-Mangiferin) and analysed the mechanism of its potent anti-inflammatory effects in LPS stimulated RAW 264.7 mouse macrophage cell line and in a carrageenan induced air pouch model. PT-Mangiferin was non-toxic to primary cells but showed significant toxicity and apoptotic effect on cancerous cells. It significantly reduced the production of pro-inflammatory mediators (COX-2, iNOS and TNF-α) in LPS stimulated RAW 264.7 cells. Further, it has also reduced the generation of ROS and inhibited LPS induced NF-kB translocation in these cells. Additionally, PT-Mangiferin significantly reduced inflammation in a mouse air pouch model by inhibiting the infiltration of monocytes and neutrophils and reducing the production of cytokines. These effects were mediated via inactivation of NLRP3 inflammasome complex and its downstream signalling molecules. Taken together these results suggest that PT-Mangiferin is potent anti-inflammatory compound that reduces inflammation and holds promise in development of herbal based anti-inflammatory therapeutics in future.
最近的报告表明，植物成分在调节炎症反应方面发挥着作用。从 Mangifera indica 中分离出的 Mangiferin 具有很强的抗氧化、抗糖尿病和抗炎活性。然而，其抗炎活性的分子机制尚未得到很好的理解。在这项研究中，我们从野葛（Pueraria tuberosa）的块茎中分离出 Mangiferin（PT-Mangiferin），并分析了其在 LPS 刺激的 RAW 264.7 小鼠巨噬细胞系和角叉菜胶诱导的气囊模型中发挥强大抗炎作用的机制。PT-Mangiferin 对原代细胞无毒，但对癌细胞有明显的毒性和凋亡作用。它显著降低了 LPS 刺激的 RAW 264.7 细胞中促炎介质（COX-2、iNOS 和 TNF-α）的产生。此外，它还减少了 ROS 的产生，并抑制了 LPS 诱导的这些细胞中 NF-kB 的易位。此外，PT-Mangiferin 通过抑制单核细胞和中性粒细胞的浸润以及减少细胞因子的产生，显著减轻了小鼠气囊模型中的炎症。这些作用是通过 NLRP3 炎性小体复合物及其下游信号分子的失活介导的。综上所述，这些结果表明，PT-Mangiferin 是一种有效的抗炎化合物，可减轻炎症，有望在未来开发基于草药的抗炎治疗方法。
