Saclay Plant Science, Institut de Biologie des Plantes, Univ. Paris-Sud, CNRS, Orsay, 91405, France.
GQE- Le Moulon, INRA, Univ. Paris-Sud, CNRS, AgroParisTech, Université Paris-Saclay, Gif-sur-Yvette, 91190, France.
Plant J. 2017 May;90(3):560-572. doi: 10.1111/tpj.13513. Epub 2017 Mar 26.
Procambial and cambial stem cells provide the initial cells that allow the formation of vascular tissues. WOX4 and WOX14 have been shown to act redundantly to promote procambial cell proliferation and differentiation. Gibberellins (GAs), which have an important role in wood formation, also stimulate cambial cell division. Here we show that the loss of WOX14 function phenocopies some traits of GA-deficient mutants that can be complemented by exogenous GA application, whereas WOX14 overexpression stimulates the expression of GA3ox anabolism genes and represses GA2ox catabolism genes, promoting the accumulation of bioactive GA. More importantly, our data clearly indicate that WOX14 but not WOX4 promotes vascular cell differentiation and lignification in inflorescence stems of Arabidopsis.
原形成层和形成层干细胞为维管组织的形成提供了最初的细胞。WOX4 和 WOX14 被证明具有促进原形成层细胞增殖和分化的冗余作用。赤霉素（GA）在木材形成中具有重要作用，也刺激形成层细胞分裂。在这里，我们表明 WOX14 功能的丧失模拟了一些 GA 缺陷突变体的特征，这些特征可以通过外源 GA 应用来弥补，而 WOX14 的过表达刺激了 GA3ox 合成代谢基因的表达，并抑制了 GA2ox 分解代谢基因的表达，促进了生物活性 GA 的积累。更重要的是，我们的数据清楚地表明，WOX14 而不是 WOX4 促进了拟南芥花序茎中血管细胞的分化和木质化。
