HERC4的RNA干扰抑制宫颈癌Hela细胞的增殖、凋亡和迁移
[RNA interference of HERC4 inhibits proliferation, apoptosis and migration of cervical cancer Hela cells].
作者信息
Wei Min, Zhang Yan-Ling, Chen Lan, Cai Cui-Xia, Wang Han-Duo
机构信息
Clinical Laboratory, Nanshan Maternity Child Healthcare Hospital, Shenzhen 518067, China. E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2016 Feb 20;37(2):232-237. doi: 10.3969/j.issn.1673-4254.2017.02.15.
OBJECTIVE
To explore the effects of silencing HERC4 on the proliferation, apoptosis, and migration of cervical cancer cell line Hela and the possible molecular mechanisms.
METHODS
Three HERC4-specific small interfering RNAs (siRNAs) were transfected into Hela cells, and HERC4 expression in the cells was examined with Western blotting. CCK-8 assay, annexin V-FITC/PI assay, and wound healing assay were used to assess the effect of HERC4 silencing on the proliferation, apoptosis and migration ability of Hela cells. The expression levels of cyclin D1 and Bcl-2 in the cells were detected using Western blotting.
RESULTS
Transfection of siRNA-3 resulted in significantly decreased HERC4 protein expression (P<0.01). HERC4 silencing by siRNA-3 markedly suppressed the proliferation and migration of Hela cells, increased the apoptosis rate (P<0.01) and reduced the expression levels of cyclin D1 and Bcl-2 (P<0.01).
CONCLUSION
Silencing of HERC4 efficiently inhibits the proliferation, migration, and invasion of Hela cells in vitro, and the underlying mechanisms may involve the down-regulation of cyclin D1 and Bcl-2.
目的
探讨沉默HERC4对宫颈癌Hela细胞系增殖、凋亡及迁移的影响及其可能的分子机制。
方法
将3种HERC4特异性小干扰RNA（siRNAs）转染至Hela细胞，采用蛋白质免疫印迹法检测细胞中HERC4的表达。采用CCK-8法、膜联蛋白V-FITC/PI法及划痕愈合实验评估沉默HERC4对Hela细胞增殖、凋亡及迁移能力的影响。采用蛋白质免疫印迹法检测细胞中细胞周期蛋白D1及Bcl-2的表达水平。
结果
转染siRNA-3后HERC4蛋白表达显著降低（P<0.01）。siRNA-3沉默HERC4可显著抑制Hela细胞的增殖及迁移，增加凋亡率（P<0.01），并降低细胞周期蛋白D1及Bcl-2的表达水平（P<0.01）。
结论
沉默HERC4可有效抑制体外培养的Hela细胞的增殖、迁移及侵袭，其潜在机制可能与细胞周期蛋白D1及Bcl-2表达下调有关。
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