Bou Gerelchimeg, Liu Shichao, Sun Mingju, Zhu Jiang, Xue Binghua, Guo Jia, Zhao Yueming, Qu Bo, Weng Xiaogang, Wei Yanchang, Lei Lei, Liu Zhonghua
College of Life Science, Northeast Agricultural University, Harbin 150030, China.
College of Animal Science, Inner Mongolia Agricultural University, Huhhot 010018, China.
Development. 2017 Apr 1;144(7):1296-1306. doi: 10.1242/dev.141085. Epub 2017 Feb 20.
The role of CDX2 in trophectoderm (TE) cells has been extensively studied, yet the results are contradictory and species specific. Here, CDX2 expression and function were explored in early porcine embryos. Notably, siRNA-mediated gene knockdown and lentivirus-mediated TE-specific gene regulation demonstrated that CDX2 is essential for the maintenance of blastocyst integrity by regulating the BMP4-mediated blastocyst niche and classic protein kinase C (PKC)-mediated TE polarity in mammalian embryos. Mechanistically, -depleted porcine embryos stalled at the blastocyst stage and exhibited apoptosis and inactive cell proliferation, possibly resulting from BMP4 downregulation. Moreover, TE cells in -depleted blastocysts displayed defective F-actin apical organization associated with downregulation of PKCα (). Collectively, these results provide further insight into the functional diversity of CDX2 in early mammalian embryos.
CDX2在滋养外胚层（TE）细胞中的作用已得到广泛研究，但其结果相互矛盾且具有物种特异性。在此，对早期猪胚胎中的CDX2表达和功能进行了探究。值得注意的是，小干扰RNA介导的基因敲低和慢病毒介导的TE特异性基因调控表明，CDX2通过调节哺乳动物胚胎中骨形态发生蛋白4（BMP4）介导的胚泡微环境和经典蛋白激酶C（PKC）介导的TE极性，对维持胚泡完整性至关重要。从机制上讲，CDX2缺失的猪胚胎停滞在胚泡阶段，并表现出细胞凋亡和细胞增殖不活跃，这可能是由于BMP4下调所致。此外，CDX2缺失的胚泡中的TE细胞显示出与PKCα下调相关的F-肌动蛋白顶端组织缺陷。总体而言，这些结果为深入了解CDX2在早期哺乳动物胚胎中的功能多样性提供了进一步的依据。
