马皮肤肉瘤与健康皮肤样本DNA甲基化模式的比较分析。
Comparative analysis of DNA methylation patterns of equine sarcoid and healthy skin samples.
作者信息
Semik E, Ząbek T, Gurgul A, Fornal A, Szmatoła T, Pawlina K, Wnuk M, Klukowska-Rötzler J, Koch C, Mählmann K, Bugno-Poniewierska M
机构信息
Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Balice, Poland.
Department of Genetics, Centre of Applied Biotechnology and Basic Sciences, University of Rzeszow, Rzeszow, Poland.
出版信息
Vet Comp Oncol. 2018 Mar;16(1):37-46. doi: 10.1111/vco.12308. Epub 2017 Feb 21.
OBJECTIVE
In this study, for the first time we report the genome-wide DNA methylation profile of skin tumour in horses and describe differentially methylated genomic regions (DMRs) with respect to healthy skin.
MATERIALS & METHODS: The comparative analysis of DNA methylation patterns detected using Reduced Representation Bisulfite Sequencing (RRBS) technique, allowed identification of 136 regions showing differential methylation between sarcoid and normal skin tissue.
RESULTS
Most of the identified DMRs were short fragments, less than 1 kb in size, located in the intergenic regions. Among identified DMRs there were also regions located within genes directly or indirectly related with oncogenesis. We additionally validated 9 CpG sites showing hypomethylation and 9 CpG sites that were hypermethylated in lesional sample, confirming the identified changes in the DNA methylation.
CONCLUSION
Knowledge on the changes taking place in the process of DNA methylation may provide a basis for the development of new alternative diagnostic or therapeutic approaches to equine sarcoids.
目的
在本研究中，我们首次报告马皮肤肿瘤的全基因组DNA甲基化谱，并描述相对于健康皮肤的差异甲基化基因组区域（DMRs）。
材料与方法
使用简化代表性亚硫酸氢盐测序（RRBS）技术对检测到的DNA甲基化模式进行比较分析，从而鉴定出136个在肉瘤组织和正常皮肤组织之间显示出差异甲基化的区域。
结果
大多数鉴定出的DMRs是短片段，大小小于1 kb，位于基因间区域。在鉴定出的DMRs中，也有位于与肿瘤发生直接或间接相关的基因内的区域。我们还验证了9个在病变样本中显示低甲基化的CpG位点和9个高甲基化的CpG位点，证实了DNA甲基化中已确定的变化。
结论
关于DNA甲基化过程中发生的变化的知识可能为开发针对马肉瘤的新的替代诊断或治疗方法提供基础。
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