Key Laboratory for Advanced Materials & Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, P. R. China.
ACS Appl Mater Interfaces. 2017 Mar 15;9(10):8498-8507. doi: 10.1021/acsami.6b15599. Epub 2017 Mar 2.
Photoresponsive smart surfaces are promising candidates for a variety of applications in optoelectronics and sensing devices. The use of light as an order signal provides advantages of remote and noninvasive control with high temporal and spatial resolutions. Modification of the photoswitches with target biomacromolecules, such as peptides, DNA, and small molecules including folic acid derivatives and sugars, has recently become a popular strategy to empower the smart surfaces with an improved detection efficiency and specificity. Herein, we report the construction of photoswitchable self-assembled monolayers (SAMs) based on sugar (galactose/mannose)-decorated azobenzene derivatives and determine their photoswitchable, selective protein/cell adhesion performances via electrochemistry. Under alternate UV/vis irradiation, interconvertible high/low recognition and binding affinity toward selective lectins (proteins that recognize sugars) and cells that highly express sugar receptors are achieved. Furthermore, the cis-SAMs with a low binding affinity toward selective proteins and cells also exhibit minimal response toward unselective protein and cell samples, which offers the possibility in avoiding unwanted contamination and consumption of probes prior to functioning for practical applications. Besides, the electrochemical technique used facilitates the development of portable devices based on the smart surfaces for on-demand disease diagnosis.
光响应智能表面在光电子学和传感设备的各种应用中具有广阔的应用前景。利用光作为控制信号，具有远程、非侵入性、高时空分辨率的优点。通过将光开关与目标生物大分子（如肽、DNA 以及包括叶酸衍生物和糖在内的小分子）进行修饰，最近已成为赋予智能表面更高检测效率和特异性的一种热门策略。本研究报道了基于糖（半乳糖/甘露糖）修饰的偶氮苯衍生物构建光响应自组装单层（SAMs），并通过电化学确定了其光响应、选择性蛋白/细胞黏附性能。在交替的紫外/可见光照下，实现了对选择性凝集素（识别糖的蛋白质）和高度表达糖受体的细胞的高/低识别和结合亲和力的相互转换。此外，对选择性蛋白和细胞具有低结合亲和力的顺式-SAMs 对非选择性蛋白和细胞样本的响应也最小，这为实际应用中避免探针的非预期污染和消耗提供了可能。此外，所使用的电化学技术有利于开发基于智能表面的便携式设备，用于按需疾病诊断。
