氟对中性粒细胞腺苷酸环化酶的双重调节及其与细胞活化的关系。
Dual regulation of neutrophil adenylate cyclase by fluoride and its relationship to cellular activation.
作者信息
Saad M, Strnad C F, Wong K
机构信息
Department of Pharmacology, University of Alberta, Edmonton, Canada.
出版信息
Br J Pharmacol. 1987 Aug;91(4):715-9. doi: 10.1111/j.1476-5381.1987.tb11268.x.
1 Fluoride stimulated (1-10 mM) and inhibited (10-100 mM) adenylate cyclase of neutrophil membranes in a GTP-independent manner. The latter fluoride concentration range corresponded to that shown previously to induce cellular responses. 2 Dual regulation of cyclase activity was also exhibited by a nonhydrolysable GTP analogue, guanosine 5'[gamma-thio]triphosphate (GTP gamma S). Inhibition was observed at 0.1-10 nM GTP gamma S while stimulation occurred at greater than 10 nM GTP gamma S. 3 Relatively high levels (greater than microM) of formylmethionyl-leucyl-phenylalanine inhibited adenylate cyclase in the presence of GTP (10 microM). 4 Pertussis toxin pretreatment abolished adenylate cyclase inhibition mediated by GTP gamma S and formylmethionyl-leucyl-phenylalanine but did not influence fluoride-induced inhibition.
- 氟化物（1 - 10 mM）以不依赖GTP的方式刺激中性粒细胞膜的腺苷酸环化酶，而（10 - 100 mM）则抑制该酶。后一氟化物浓度范围与先前显示可诱导细胞反应的浓度范围相对应。2. 一种不可水解的GTP类似物，鸟苷5'-[γ-硫代]三磷酸（GTPγS）也表现出对环化酶活性的双重调节。在0.1 - 10 nM GTPγS时观察到抑制作用，而在大于10 nM GTPγS时出现刺激作用。3. 相对高水平（大于微摩尔）的甲酰甲硫氨酰 - 亮氨酰 - 苯丙氨酸在存在GTP（10 microM）的情况下抑制腺苷酸环化酶。4. 百日咳毒素预处理消除了由GTPγS和甲酰甲硫氨酰 - 亮氨酰 - 苯丙氨酸介导的腺苷酸环化酶抑制作用，但不影响氟化物诱导的抑制作用。
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