Kurata S, Kurata N, Ikawa Y
Department of Biochemical Genetics, Tokyo Medical and Dental University, Japan.
Cancer Res. 1987 Nov 15;47(22):5908-12.
A simple oncogene isolation was proved using the SD1-T rat embryonic cell line. The SD1-T cell line, which releases endogenous rat leukemia virus, was cocultured with (a) normal rat kidney cells transformed by cloned v-mos DNA, (b) the rat mammary tumor cell line (63SP), or (c) normal rat kidney cells transformed by 63SP DNA. Within 1 mo, oncogenic viruses were recovered from all three coculture supernatants. During this period, increase of oncogenic transcripts was observed in the cocultured cells. The oncogenic viruses appeared to contain the mos gene in cocultures (a) and ras-related sequences in cocultures (b) and (c). The emergence of virus containing mos from mos DNA-mediated normal rat kidney transformants demonstrated "rescue" of the active cellular oncogene by the rat leukemia virus. This coculture system seems to facilitate "rescue" of oncogenes functioning in the tumor and transformed cells.
利用SD1-T大鼠胚胎细胞系证明了一种简单的癌基因分离方法。释放内源性大鼠白血病病毒的SD1-T细胞系与以下细胞共培养：(a) 由克隆的v-mos DNA转化的正常大鼠肾细胞，(b) 大鼠乳腺肿瘤细胞系(63SP)，或(c) 由63SP DNA转化的正常大鼠肾细胞。在1个月内，从所有三种共培养上清液中回收了致癌病毒。在此期间，在共培养细胞中观察到致癌转录本增加。致癌病毒在共培养物(a)中似乎含有mos基因，在共培养物(b)和(c)中含有ras相关序列。从mos DNA介导的正常大鼠肾转化体中出现含mos的病毒，证明大鼠白血病病毒对活性细胞癌基因的“拯救”。这种共培养系统似乎有助于“拯救”在肿瘤和转化细胞中起作用的癌基因。
