Faculty of Oral and Dental Medicine, Cairo University, 11222, Egypt.
National Cancer Institute, Cairo University, Egypt.
Arch Oral Biol. 2017 Jun;78:94-99. doi: 10.1016/j.archoralbio.2017.02.015. Epub 2017 Feb 14.
Differentiation between the aggressive benign odontogenic tumors and their malignant counterparts is controversial and difficult. While flow cytometry (FCM) allowed DNA analysis in neoplasia, argyrophilic organizer regions (AgNORs) number and/or size in a nucleus are correlated with the ribosomal gene activity and therefore with cellular proliferation. The aim of this research was to study the diagnostic accuracy of FCM and AgNORs staining in differentiating between benign and malignant epithelial odontogenic tumors and to correlate between these two interventions.
Sixteen benign cases [8 cases of ameloblastoma (AB) and 8 cases of keratocystic odontogenic tumor (KCOT)] and 13 malignant epithelial odontogenic tumors [8 cases of ameloblastic carcinoma (ABC) and 5 cases of clear cell odontogenic carcinoma(CCOC)] were included in the current study. For FCM analysis, a single cell suspension from Formalin fixed paraffin-embedded (FFPE) tumors was prepared according to a modified method described by Hedley (1989) and AgNORs staining were performed in accordance to the Ploton protocol (1986). Analysis of AgNORs was performed using both quantitative and qualitative methods.
The work revealed that all the examined tumors were diploid, except for 40% of CCOC cases. The S-phase fraction (SPF) value, AgNORs count and AgNORs area/cell showed statistically significant difference on comparing benign and malignant groups. A weak positive correlation was observed between SPF and AgNORs count.
The SPF value was considered to be more sensitive and specific in differentiation between aggressive benign and malignant epithelial odontogenic tumors in comparison to AgNORs counting.
区分侵袭性良性牙源性肿瘤与其恶性对应物具有争议且困难。虽然流式细胞术（FCM）允许对肿瘤进行 DNA 分析，但核内嗜银结构组织者区域（AgNORs）的数量和/或大小与核糖体基因活性相关，因此与细胞增殖相关。本研究旨在研究 FCM 和 AgNORs 染色在区分良性和恶性上皮性牙源性肿瘤中的诊断准确性，并对这两种干预措施进行相关性研究。
本研究纳入了 16 例良性病例（8 例造釉细胞瘤[AB]和 8 例角化囊肿性牙源性肿瘤[KCOT]）和 13 例恶性上皮性牙源性肿瘤（8 例成釉细胞瘤[ABC]和 5 例透明细胞牙源性癌[CCOC]）。为了进行 FCM 分析，根据 Hedley（1989 年）描述的改良方法从福尔马林固定石蜡包埋（FFPE）肿瘤中制备单细胞悬液，并按照 Ploton 方案（1986 年）进行 AgNORs 染色。使用定量和定性方法分析 AgNORs。
结果显示，除了 40%的 CCOC 病例外，所有检查的肿瘤均为二倍体。S 期分数（SPF）值、AgNORs 计数和 AgNORs 面积/细胞在良性和恶性组之间的比较具有统计学显著差异。SPF 与 AgNORs 计数之间观察到弱正相关。
与 AgNORs 计数相比，SPF 值在区分侵袭性良性和恶性上皮性牙源性肿瘤方面被认为更敏感和特异。
