Serrano Isa, Oliveira Manuela, Santos José Pedro, Bilocq Florence, Leitão Alexandre, Tavares Luis, Pirnay Jean-Paul, De Vos Daniel
CIISA/Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Brussels, Belgium.
BMC Vet Res. 2017 Feb 21;13(1):58. doi: 10.1186/s12917-017-0977-8.
Pseudomonas aeruginosa is an important human opportunistic pathogen responsible for fatal nosocomial infections worldwide, and has emerged as a relevant animal pathogen. Treatment options are dramatically decreasing, due to antimicrobial resistance and the microorganism's large versatile genome. Antimicrobial resistance profiles, serotype frequency and genomic profile of unrelated P. aeruginosa isolates of veterinary origin (n = 73), including domesticated, farm, zoo and wild animals mainly from Portugal were studied. The genomic profile, determined by DiversiLab system (Rep-PCR-based technique), was compared with the P. aeruginosa global population structure to evaluate their relatedness.
Around 40% of the isolates expressed serotypes O6 (20.5%) and O1 (17.8%). A total of 46.6% of isolates was susceptible to all antimicrobials tested. Isolates obtained from most animals were non-multidrug resistant (86.3%), whereas 11% were multidrug resistant, MDR (non-susceptible to at least one agent in ≥ three antimicrobial categories), and 2.7% extensively drug resistant, XDR (non-susceptible to at least one agent in all but two or fewer antimicrobial categories). Resistance percentages were as follows: amikacin (0.0%), aztreonam (41.1%), cefepime (9.6%), ceftazidime (2.7%), ciprofloxacin (15.1%), colistin (0.0%), gentamicin (12.3%), imipenem (1.4%), meropenem (1.4%), piperacillin + tazobactam (12.3%), ticarcillin (16.4%), ticarcillin + clavulanic acid (17.8%), and tobramycin (1.4%). Animal isolates form a population with a non-clonal epidemic structure indistinguishable from the global P. aeruginosa population structure, where no specific 'animal clonal lineage' was detected.
Serotypes O6 and O1 were the most frequent. Serotype frequency and antimicrobial resistance patterns found in P. aeruginosa from animals were as expected for this species. This study confirms earlier results that P. aeruginosa has a non-clonal population structure, and shows that P. aeruginosa population from animals is homogeneously scattered and indistinguishable from the global population structure.
铜绿假单胞菌是一种重要的人类机会致病菌，在全球范围内导致致命的医院感染，并且已成为一种重要的动物病原体。由于抗菌药物耐药性以及该微生物庞大且多功能的基因组，治疗选择正在急剧减少。本研究对主要来自葡萄牙的兽医源无关铜绿假单胞菌分离株（n = 73）进行了研究，这些分离株来自家养动物、农场动物、动物园动物和野生动物，分析了其抗菌药物耐药谱、血清型频率和基因组图谱。通过DiversiLab系统（基于重复序列聚合酶链反应的技术）确定的基因组图谱与铜绿假单胞菌全球种群结构进行比较，以评估它们之间的亲缘关系。
约40%的分离株表达血清型O6（20.5%）和O1（17.8%）。共有46.6%的分离株对所有测试抗菌药物敏感。从大多数动物获得的分离株是非多重耐药的（86.3%），而11%是多重耐药的（MDR，对至少三类抗菌药物中的至少一种药物不敏感），2.7%是广泛耐药的（XDR，对除两类或更少类抗菌药物外的所有药物中的至少一种药物不敏感）。耐药百分比如下：阿米卡星（0.0%）、氨曲南（41.1%）、头孢吡肟（9.6%）、头孢他啶（2.7%）、环丙沙星（15.1%）、黏菌素（0.0%）、庆大霉素（12.3%）、亚胺培南（1.4%）、美罗培南（1.4%）、哌拉西林 + 他唑巴坦（12.3%）、替卡西林（16.4%）、替卡西林 + 克拉维酸（17.8%）和妥布霉素（1.4%）。动物分离株形成了一个具有非克隆流行结构的种群，与全球铜绿假单胞菌种群结构无法区分，未检测到特定的“动物克隆谱系”。
血清型O6和O1最为常见。动物来源的铜绿假单胞菌中发现的血清型频率和抗菌药物耐药模式与该物种预期的情况一致。本研究证实了早期的结果，即铜绿假单胞菌具有非克隆种群结构，并表明动物来源的铜绿假单胞菌种群均匀分布且与全球种群结构无法区分。
