Panzarini Elisa, Mariano Stefania, Vergallo Cristian, Carata Elisabetta, Fimia Gian Maria, Mura Francesco, Rossi Marco, Vergaro Viviana, Ciccarella Giuseppe, Corazzari Marco, Dini Luciana
Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, Lecce, Italy.
Department of Base and Applied Science to Engineering, Sapienza University of Rome, Rome, Italy.
Toxicol In Vitro. 2017 Jun;41:64-74. doi: 10.1016/j.tiv.2017.02.014. Epub 2017 Feb 20.
This study aims to determine the interaction (uptake and biological effects on cell viability and cell cycle progression) of glucose capped silver nanoparticles (AgNPs-G) on human epithelioid cervix carcinoma (HeLa) cells, in relation to amount, 2×10 or 2×10 NPs/cell, and exposure time, up to 48h. The spherical and well dispersed AgNPs (30±5nm) were obtained by using glucose as reducing agent in a green synthesis method that ensures to stabilize AgNPs avoiding cytotoxic soluble silver ions Ag release. HeLa cells take up abundantly and rapidly AgNPs-G resulting toxic to cells in amount and incubation time dependent manner. HeLa cells were arrested at S and G2/M phases of the cell cycle and subG1 population increased when incubated with 2×10 AgNPs-G/cell. Mitotic index decreased accordingly. The dissolution experiments demonstrated that the observed effects were due only to AgNPs-G since glucose capping prevents Ag release. The AgNPs-G influence on HeLa cells viability and cell cycle progression suggest that AgNPs-G, alone or in combination with chemotherapeutics, may be exploited for the development of novel antiproliferative treatment in cancer therapy. However, the possible influence of the cell cycle on cellular uptake of AgNPs-G and the mechanism of AgNPs entry in cells need further investigation.
本研究旨在确定葡萄糖包被的银纳米颗粒（AgNPs-G）与人上皮样宫颈癌（HeLa）细胞之间的相互作用（摄取以及对细胞活力和细胞周期进程的生物学效应），这与剂量（2×10或2×10个颗粒/细胞）和暴露时间（长达48小时）有关。通过在绿色合成方法中使用葡萄糖作为还原剂获得了球形且分散良好的AgNPs（30±5nm），该方法可确保稳定AgNPs，避免细胞毒性可溶性银离子Ag的释放。HeLa细胞大量且快速摄取AgNPs-G，其对细胞产生的毒性呈剂量和孵育时间依赖性。当与2×10个AgNPs-G/细胞一起孵育时，HeLa细胞停滞在细胞周期的S期和G2/M期，亚G1期细胞群体增加。有丝分裂指数相应降低。溶解实验表明，观察到的效应仅归因于AgNPs-G，因为葡萄糖包被可防止Ag释放。AgNPs-G对HeLa细胞活力和细胞周期进程的影响表明，AgNPs-G单独或与化疗药物联合使用，可能被用于开发癌症治疗中的新型抗增殖疗法。然而，细胞周期对AgNPs-G细胞摄取的可能影响以及AgNPs进入细胞的机制需要进一步研究。
