Douglas Timothy E L, Sobczyk Katarzyna, Łapa Agata, Włodarczyk Katarzyna, Brackman Gilles, Vidiasheva Irina, Reczyńska Katarzyna, Pietryga Krzysztof, Schaubroeck David, Bliznuk Vitaliy, Voort Pascal Van Der, Declercq Heidi A, Bulcke Jan Van den, Samal Sangram Keshari, Khalenkow Dmitry, Parakhonskiy Bogdan V, Van Acker Joris, Coenye Tom, Lewandowska-Szumieł Małgorzata, Pamuła Elżbieta, Skirtach Andre G
Department of Molecular Biotechology, Ghent University, Coupure Links 653, B-9000 Gent, Belgium. Engineering Department, Lancaster University, United Kingdom.
Biomed Mater. 2017 Mar 24;12(2):025015. doi: 10.1088/1748-605X/aa6200.
Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase. In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg. Zn and Ca were incorporated into microparticle preparations to a greater extent than Mg. Microparticle groups were heterogeneous and contained microparticles of differing shape and elemental composition. Zn-rich microparticles were 'star shaped' and appeared to consist of small crystallites, while Zn-poor, Ca- and Mg-rich microparticles were irregular in shape and appeared to contain lager crystallites. Zn-free microparticle groups exhibited the best cytocompatibility and, unexpectedly, Zn-free composites showed the highest antibacterial activity towards methicilin-resistant Staphylococcus aureus. Composites containing Zn-free microparticles were cytocompatible and therefore appear most suitable for applications as an injectable biomaterial. This study proves the principle of creating bi- and tri-elemental microparticles to induce the gelation of GG to create injectable hydrogel-microparticle composites.
用于组织再生的可注射复合材料可通过将无机微粒和细胞分散在水凝胶相中制备。在本研究中，将含有不同量钙、镁和锌的多功能碳酸盐微粒与阴离子多糖结冷胶（GG）溶液混合，以形成含锌、钙和镁的可注射水凝胶-微粒复合材料。锌和钙比镁更多地掺入微粒制剂中。微粒组是异质的，包含形状和元素组成不同的微粒。富含锌的微粒呈“星形”，似乎由小晶体组成，而贫锌、富含钙和镁的微粒形状不规则，似乎包含更大的晶体。无锌微粒组表现出最佳的细胞相容性，出乎意料的是，无锌复合材料对耐甲氧西林金黄色葡萄球菌表现出最高的抗菌活性。含有无锌微粒的复合材料具有细胞相容性，因此似乎最适合用作可注射生物材料。本研究证明了制备二元和三元微粒以诱导结冷胶凝胶化从而制备可注射水凝胶-微粒复合材料的原理。
