Chegini N, Rao C V
Department of Obstetrics and Gynecology, University of Louisvelle School of Medicine, Kentucky 40292.
Endocrinology. 1987 Nov;121(5):1870-8. doi: 10.1210/endo-121-5-1870.
A group of electron-dense granules of 150-300 nm in diameter, with a single limiting membrane, were found adjacent to the nuclei of large (25-50 microns in diameter), but not small (15-22 microns), bovine luteal cells of pregnancy. These granules were quite heterogeneous with respect to size, shape, content, etc. Most granules contained varying amounts of light and dark electron-dense material, and very few contained only light or dark material in their core. These granules occupied 160.4 +/- 7.8 microns3 cytoplasmic volume, which did not significantly decrease until 2 h of incubation without any hormone at 22 or 38 C (P greater than 0.05). Subsequently, however, volume occupancy of these granules decreased to 127.3 +/- 6.4 and 117.6 +/- 8.0 microns3 (P less than 0.05) by 4 and 12 h of incubation, respectively. Incubation of luteal tissue with 10 nM prostaglandin F2 alpha (PGF2 alpha), on the other hand, resulted in a decrease to 124.4 +/- 8.0 microns3 of volume occupancy of granules by 10 min (P less than 0.05). The volume occupancy further decreased to 46.2 +/- 4.8 microns3 by 2 h (P less than 0.01), and then the granules virtually disappeared from luteal cells. While PGF2 alpha decreased volume occupancy of granules similarly at 22 and 38 C, it had no effect at 4 C. PGF2 alpha decreased volume occupancy of granules at 0.01 nM (P less than 0.05), which continued to decrease with increasing PGF2 alpha concentrations, reaching a maximal decrease at 10 nM PGF2 alpha (P less than 0.05). PGE1, hCG, human LH, human PRL, or (Bu)2cAMP had no effect on the granules, suggesting that the PGF2 alpha effect was hormone specific. PGE1 and hCG, which protect corpus luteum from the luteolytic action of PGF2 alpha, could not inhibit degranulation by PGF2 alpha. These results demonstrate that treatment with PGF2 alpha in vitro results in a decrease in volume occupancy of nuclear associated granules in bovine large luteal cells of pregnancy in a time-, temperature-, and concentration-dependent and hormone-specific manner.
在妊娠牛的大黄体细胞（直径25 - 50微米）而非小黄体细胞（直径15 - 22微米）的细胞核附近，发现了一组直径为150 - 300纳米的电子致密颗粒，这些颗粒有一层单一的界膜。这些颗粒在大小、形状、内容物等方面差异很大。大多数颗粒含有不同量的浅色和深色电子致密物质，很少有颗粒在其核心仅含有浅色或深色物质。这些颗粒占据的细胞质体积为160.4±7.8立方微米，在22℃或38℃无任何激素孵育2小时之前，该体积没有显著减少（P＞0.05）。然而，随后，这些颗粒的体积占有率在孵育4小时和12小时时分别降至127.3±6.4和117.6±8.0立方微米（P＜0.05）。另一方面，用10 nM前列腺素F2α（PGF2α）孵育黄体组织，10分钟内颗粒的体积占有率降至124.4±8.0立方微米（P＜0.05）。到2小时时，体积占有率进一步降至46.2±4.8立方微米（P＜0.01），然后颗粒实际上从黄体细胞中消失。虽然PGF2α在22℃和38℃时对颗粒体积占有率的降低作用相似，但在4℃时无作用。PGF2α在0.01 nM时可降低颗粒的体积占有率（P＜0.05），随着PGF2α浓度的增加，其体积占有率持续降低，在10 nM PGF2α时达到最大降低幅度（P＜0.05）。前列腺素E1、人绒毛膜促性腺激素、人促黄体生成素、人催乳素或双丁酰环磷腺苷对这些颗粒无影响，这表明PGF2α的作用具有激素特异性。前列腺素E1和人绒毛膜促性腺激素可保护黄体免受PGF2α的溶黄体作用，但不能抑制PGF2α诱导的脱颗粒。这些结果表明，体外使用PGF2α处理会导致妊娠牛大黄体细胞核相关颗粒的体积占有率以时间、温度、浓度依赖性和激素特异性的方式降低。
