Lv Dan, Guo Kai-Wen, Xu Chan, Huang Mi, Zheng Si-Jian, Ma Xin-Hua, Pan Li-Hong, Wang Qiang, Yang Xin-Zhou
School of Medicine, Wuhan University of Science and Technology, Wuhan, 430065, China.
School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, 430074, China.
J Huazhong Univ Sci Technolog Med Sci. 2017 Feb;37(1):87-92. doi: 10.1007/s11596-017-1699-7. Epub 2017 Feb 22.
Siegesbeckia pubescens (SP) has been used as a traditional medicine for the treatment of and inflammatory diseases. However, the activities of SP against hepatocellular carcinoma and the related mechanisms remain unclear. The present study aimed to examine the effects of the essential oil of SP (SPEO) on the proliferation of hepatocellular carcinoma cells and the possible mechanisms. The growth inhibition of HepG2 cells was analyzed by MTT assay. Hoechst 33258 and fluorescence microscopy were utilized to observe the nuclear morphological changes of apoptotic cells. Flow cytometry was used to detect cell apoptosis and cell cycle. The expressions of the target proteins were detected by Western blotting. The results showed that SPEO obviously inhibited the proliferation of HepG2 cells in a dose-dependent manner. SPEO activated a series of apoptotic proteins in HepG2 cells, increasing expression levels of Bax, caspase-3 and caspase-9, and decreasing the bcl-2 expression level. SPEO displayed promising anti-hepatocellular carcinoma activities in vitro, partly by inducing apoptosis in HepG2 cells through activating the mitochondrial pathway.
毛梗稀莶已被用作治疗炎症性疾病的传统药物。然而，毛梗稀莶对肝细胞癌的作用及其相关机制仍不清楚。本研究旨在探讨毛梗稀莶精油（SPEO）对肝癌细胞增殖的影响及其可能机制。采用MTT法分析HepG2细胞的生长抑制情况。利用Hoechst 33258和荧光显微镜观察凋亡细胞的核形态变化。采用流式细胞术检测细胞凋亡和细胞周期。通过蛋白质免疫印迹法检测靶蛋白的表达。结果表明，SPEO能明显抑制HepG2细胞的增殖，且呈剂量依赖性。SPEO激活了HepG2细胞中的一系列凋亡蛋白，增加了Bax、半胱天冬酶-3和半胱天冬酶-9的表达水平，降低了bcl-2的表达水平。SPEO在体外显示出有前景的抗肝癌活性，部分是通过激活线粒体途径诱导HepG2细胞凋亡实现的。
