黑鲪IgM单克隆抗体的制备及其应用
Development and application of monoclonal antibodies against IgM of black rockfish Sebastes schlegeli.
作者信息
Zhang W, Tang X Q, Sheng X Z, Xing J, Zhan W B
机构信息
Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, Qingdao 266003, P. R. China.
Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, No.1 Wenhai Road, Aoshanwei Town, Jimo, Qingdao, China.
出版信息
J Fish Biol. 2017 Apr;90(4):1668-1675. doi: 10.1111/jfb.13279. Epub 2017 Feb 21.
Monoclonal antibodies (mAbs) against black rockfish Sebastes schlegeli serum immunoglobulin M (IgM) were developed, which showed a specific reaction with the heavy chain of S. schlegeli IgM in Western blotting and with surface IgM positive (sIgM ) lymphocytes in indirect immunofluorescence. mAb 2A6 was employed to investigate the antibody and sIgM lymphocyte responses of S. schlegeli injected with inactivated Edwardsiella tarda, by ELISA and flow cytometry. Compared with controls, the level of specific antibodies and the percentage of sIgM lymphocytes both increased in the immunized fish and simultaneously reached their peaks at day 35 after immunization.
制备了抗黑鲪血清免疫球蛋白M（IgM）的单克隆抗体（mAb），其在蛋白质免疫印迹中与黑鲪IgM重链呈特异性反应，在间接免疫荧光中与表面IgM阳性（sIgM）淋巴细胞呈特异性反应。采用单克隆抗体2A6，通过酶联免疫吸附测定法（ELISA）和流式细胞术，研究注射灭活迟缓爱德华氏菌的黑鲪的抗体和sIgM淋巴细胞反应。与对照组相比，免疫鱼体内特异性抗体水平和sIgM淋巴细胞百分比均升高，且在免疫后第35天同时达到峰值。
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