抗小口黑鲈（Micropterus dolomieu）IgM 的小鼠单克隆抗体的制备与鉴定。
Production and characterization of a mouse monoclonal antibody against smallmouth bass (Micropterus dolomieu) IgM.
机构信息
U.S. Geological Survey, Leetown Science Center, National Fish Health Research Laboratory, 11649 Leetown Rd., Kearneysville, WV, 25430, USA.
Division of Forestry and Natural Resources, West Virginia University, 333 Evansdale Drive, Morgantown, WV, 26505, USA.
出版信息
Fish Shellfish Immunol. 2021 Jun;113:20-23. doi: 10.1016/j.fsi.2021.03.006. Epub 2021 Mar 16.
A murine monoclonal antibody (mAb, IgG2a) was produced for the detection of smallmouth bass (Micropterus dolomieu) immunoglobulin (IgM). The antibody is specific for IgM heavy chain and was shown to also recognize the Ig heavy chain of largemouth bass (Micropterus salmoides) and bluegill (Lepomis macrochirus) using Western Blot analysis of plasma from 9 teleost taxa. When applied to the analysis of smallmouth bass total plasma IgM using ELISA, the mAb was found to be effective when used in an inhibition kinetic assay.
一种用于检测小口黑鲈（Micropterus dolomieu）免疫球蛋白（IgM）的鼠源单克隆抗体（mAb，IgG2a）被制备。该抗体特异性识别 IgM 重链，通过 Western Blot 分析 9 种硬骨鱼类的血浆，结果表明该抗体也可识别大口黑鲈（Micropterus salmoides）和蓝鳃太阳鱼（Lepomis macrochirus）的 Ig 重链。当该 mAb 应用于 ELISA 分析小口黑鲈总血浆 IgM 时，在抑制动力学测定中发现该 mAb 非常有效。
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