Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Yunnan 666303, China.
College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, Henan, 466001, China.
Sci Rep. 2017 Feb 22;7:43090. doi: 10.1038/srep43090.
Recent research revealed that TERMINAL FLOWER 1 (TFL1) homologues are involved in the critical developmental process of floral initiation in several plant species. In this study, the functions of three putative TFL1 homologues (JcTFL1a, JcTFL1b and JcTFL1c) in the biofuel plant Jatropha curcas were analysed using the transgenic approach. JcTFL1b and JcTFL1c, but not JcTFL1a, could complement the TFL1 function and rescue early flowering and determinate inflorescence phenotype in tfl1-14 Arabidopsis mutant, thus suggesting that JcTFL1b and JcTFL1c may be homologues of TFL1. Transgenic Jatropha overexpressing JcTFL1a, JcTFL1b or JcTFL1c showed late flowering, whereas only JcTFL1b and JcTFL1c overexpression delayed flowering in transgenic Arabidopsis. JcTFL1b-RNAi transgenic Jatropha consistently exhibited moderately early flowering phenotype. JcFT and JcAP1 were significantly downregulated in transgenic Jatropha overexpressing JcTFL1a, JcTFL1b or JcTFL1c, which suggested that the late flowering phenotype of these transgenic Jatropha may result from the repressed expression of JcFT and JcAP1. Our results indicate that these three JcTFL1 genes play redundant roles in repressing flowering in Jatropha.
最近的研究表明，TERMINAL FLOWER 1（TFL1）同源物参与了几个植物物种中花起始的关键发育过程。在这项研究中，使用转基因方法分析了生物燃料植物麻疯树中的三个假定 TFL1 同源物（JcTFL1a、JcTFL1b 和 JcTFL1c）的功能。JcTFL1b 和 JcTFL1c，但不是 JcTFL1a，可以补充 TFL1 的功能，并挽救 tfl1-14 拟南芥突变体中的早期开花和确定花序表型，因此表明 JcTFL1b 和 JcTFL1c 可能是 TFL1 的同源物。过表达 JcTFL1a、JcTFL1b 或 JcTFL1c 的转基因麻疯树表现出晚花，而只有 JcTFL1b 和 JcTFL1c 过表达会延迟转基因拟南芥的开花。JcTFL1b-RNAi 转基因麻疯树表现出一致的中度早花表型。在过表达 JcTFL1a、JcTFL1b 或 JcTFL1c 的转基因麻疯树中，JcFT 和 JcAP1 显著下调，这表明这些转基因麻疯树的晚花表型可能是由于 JcFT 和 JcAP1 的表达受到抑制。我们的研究结果表明，这三个 JcTFL1 基因在抑制麻疯树开花中发挥冗余作用。
