携带大肠杆菌肠毒素STII基因的转座子侧翼末端序列的特征分析。

Characterization of the terminal sequences flanking the transposon that carries the Escherichia coli enterotoxin STII gene.

作者信息

Hu S T, Yang M K, Spandau D F, Lee C H

机构信息

Department of Pathology, Indiana University School of Medicine, Indianapolis 46223.

出版信息

Gene. 1987;55(2-3):157-67. doi: 10.1016/0378-1119(87)90276-9.

DOI:10.1016/0378-1119(87)90276-9

PMID:2822540

Abstract

The Escherichia coli enterotoxin STII gene is flanked by two repeat sequences, approx. 600 bp each and 8 kb apart. This 9-kb DNA fragment has been shown to transpose as a unit and is thus considered a transposon. It is presently designated as Tn4521. In this study, the two terminal sequences of Tn4521 cloned in pPS1 were localized, isolated, and characterized. The two terminal sequences were found to be composed of IS2 sequences and were in an inverted repeat orientation. However, neither repeat contained a complete IS2. The LTR contained bp 1-722, whereas the RTR contained bp 17-536 and 969-1327, all three of the IS2 sequence.

摘要

大肠杆菌肠毒素STII基因两侧有两个重复序列，每个约600 bp，相距8 kb。这个9 kb的DNA片段已被证明可作为一个单位进行转座，因此被认为是一个转座子。目前它被命名为Tn4521。在本研究中，克隆于pPS1的Tn4521的两个末端序列被定位、分离并进行了特征分析。发现这两个末端序列由IS2序列组成，且呈反向重复方向。然而，两个重复序列均不包含完整的IS2。左末端重复序列包含IS2序列的第1至722个碱基对，而右末端重复序列包含IS2序列的第17至536个碱基对以及第969至1327个碱基对。