Hozumi T, Nomura J, Ishizawa M
Int J Cancer. 1979 Jan 15;23(1):119-22. doi: 10.1002/ijc.2910230121.
Various N-substituted polymethylene diamides were synthesized and tested for their potency to induce erythroid differentiation in murine erythroleukemia cells. N,N,N',N'-tetramethyl-1.6-hexane-dicarboxamide (IIc) was the most potent inducer among 15 compounds tested. The effectiveness of this compound was similar to that of hexamethylene bisacetamide (HMBA). HMBA has a different amide linkage order from that of IIc. HMBA and IIc at a concentration of 5 mM had similar effects on the cell growth rate and induced a similar frequency of benzidine-positive cells. However, hemoglobin production was 1.5 times more effective with IIc. Polymethylene diester, diamide, dihydrazide and dianilide had no effect on the induction of hemoglobin synthesis. The N-alkylated amide group appears to be required for induction of differentiation in murine erythroleukemia cells.
合成了多种N-取代的聚亚甲基二酰胺，并测试了它们在小鼠红白血病细胞中诱导红系分化的能力。在测试的15种化合物中，N,N,N',N'-四甲基-1,6-己二酰胺（IIc）是最有效的诱导剂。该化合物的有效性与己二亚甲基双乙酰胺（HMBA）相似。HMBA的酰胺键顺序与IIc不同。浓度为5 mM时，HMBA和IIc对细胞生长速率的影响相似，并诱导出相似频率的联苯胺阳性细胞。然而，IIc对血红蛋白生成的效果要高1.5倍。聚亚甲基二酯、二酰胺、二酰肼和二苯胺对血红蛋白合成的诱导没有影响。N-烷基化酰胺基团似乎是小鼠红白血病细胞诱导分化所必需的。
