关于脊髓灰质炎减毒活疫苗（萨宾3型）的研究。
Studies on the attenuation of the Sabin type 3 oral polio vaccine.
作者信息
Almond J W, Westrop G D, Evans D M, Dunn G, Minor P D, Magrath D, Schild G C
机构信息
Department of Microbiology, University of Reading, U.K.
出版信息
J Virol Methods. 1987 Aug;17(1-2):183-9. doi: 10.1016/0166-0934(87)90081-4.
The genetic basis of attenuation of the poliovirus type 3 vaccine strain P3/Leon 12a1b has been investigated by comparing the nucleotide sequence of this strain with that of its neurovirulent progenitor P3/Leon/37 and by constructing recombinants between these two viruses using infectious cDNAs. Preliminary results suggest that attenuation is caused by just two point mutations, one occurring in the 5' non-coding region and the other causing an amino acid change in coat protein VP3.
通过将3型脊髓灰质炎疫苗株P3/Leon 12a1b的核苷酸序列与其神经毒力祖代株P3/Leon/37的序列进行比较，并利用感染性cDNA在这两种病毒之间构建重组体，对3型脊髓灰质炎疫苗株P3/Leon 12a1b减毒的遗传基础进行了研究。初步结果表明，减毒仅由两个点突变引起，一个发生在5'非编码区，另一个导致衣壳蛋白VP3中的氨基酸变化。
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