Yasukawa Takuya, Hayashi Makoto, Tanabe Natsuko, Tsuda Hiromasa, Suzuki Yusuke, Kawato Takayuki, Suzuki Naoto, Maeno Masao, Ogiso Bunnai
Department of Endodontics, Nihon University School of Dentistry.
Department of Biochemistry, Nihon University School of Dentistry.
Dent Mater J. 2017 Jul 26;36(4):469-475. doi: 10.4012/dmj.2016-313. Epub 2017 Feb 22.
Mineral trioxide aggregate (MTA) has excellent biocompatibility as well as bioactivity, including an ability to induce osteoblast differentiation. We examined the effects of the calcium-sensing receptor (CaSR) on osteogenic gene expression induced by MTA. MC3T3-E1 cells were cultured with or without (control) MTA. The expression levels of Runx2, type I collagen, and CaSR genes were analyzed by real-time polymerase chain reaction and their products were measured using enzyme-linked immunosorbent assays. The levels were increased significantly in cells exposed to MTA compared with control. Next, MC3T3-E1 cells were cultured with MTA and EGTA (a calcium chelator), because calcium ions were released continuously from MTA into the culture. Expression levels were decreased to control levels by MTA plus EGTA. NPS2143 (a CaSR antagonist) also reduced MTA-induced gene expression. These results suggest that MTA induced osteogenic gene expressions of Runx2 and type I collagen via CaSR in MC3T3-E1 cells.
矿物三氧化物凝聚体（MTA）具有出色的生物相容性和生物活性，包括诱导成骨细胞分化的能力。我们研究了钙敏感受体（CaSR）对MTA诱导的成骨基因表达的影响。MC3T3-E1细胞在有或无（对照）MTA的情况下进行培养。通过实时聚合酶链反应分析Runx2、I型胶原蛋白和CaSR基因的表达水平，并使用酶联免疫吸附测定法测量其产物。与对照相比，暴露于MTA的细胞中这些水平显著增加。接下来，MC3T3-E1细胞与MTA和乙二醇双四乙酸（EGTA，一种钙螯合剂）一起培养，因为钙离子从MTA持续释放到培养基中。MTA加EGTA使表达水平降至对照水平。NPS2143（一种CaSR拮抗剂）也降低了MTA诱导的基因表达。这些结果表明，MTA通过CaSR在MC3T3-E1细胞中诱导Runx2和I型胶原蛋白的成骨基因表达。
