Sequiera Glen Lester, Sareen Niketa, El-Rub Ejlal Abu, Dhingra Sanjiv
Institute of Cardiovascular Sciences, St-Boniface Hospital Albrechtsen Research Centre, Regenerative Medicine Program, College of Medicine, Faculty of Health Sciences, University of Manitoba, R 3028-2, 351 Tache Avenue, Winnipeg, MB, Canada, R2H2A6.
Methods Mol Biol. 2017;1553:15-23. doi: 10.1007/978-1-4939-6756-8_2.
Mesenchymal stem cells (MSCs) provide an opportunity to bring the field of regenerative medicine to realization. A lot of clinical trials are presently trying to establish their applicability in real-world scenarios. Some of the biggest challenges encountered in bringing MSCs from bench to bedside are the number of MSCs required, their procurement from various sources, and the batch-to-batch variability. This often leads to inconclusive results within and between different studies. Therefore, we have hereby proposed a simple protocol to source mesenchymal stem cells through differentiation of embryonic stem cells.
间充质干细胞（MSCs）为再生医学领域的实现提供了契机。目前许多临床试验正在尝试确立其在实际应用中的适用性。将MSCs从实验室应用到临床所面临的一些最大挑战包括所需MSCs的数量、从各种来源获取它们以及批次间的变异性。这常常导致不同研究内部以及不同研究之间的结果不确定。因此，我们在此提出了一种通过胚胎干细胞分化来获取间充质干细胞的简单方案。
