环境相关浓度壬基酚对斑马鱼性别分化的影响：多代研究。
Effect of environmentally-relevant concentrations of nonylphenol on sexual differentiation in zebrafish: a multi-generational study.
机构信息
Research Center of Hydrobiology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510632, China.
出版信息
Sci Rep. 2017 Feb 23;7:42907. doi: 10.1038/srep42907.
Nonylphenol (NP) is a persistent environmental chemical that can disrupt the organism's endocrine system, and is detected in the surface water and sea. In this study, we investigated whether NP can alter transcriptional expression of sexual differentiation-related genes. Three generations of zebrafish were exposed to 0, 2, 20 and 200 μg·L of NP, and transcriptional expression of sexual differentiation genes were assessed in 10, 20 and 40 dpf in the F1 and F2 generations. Growth of zebrafish exposed to 200 μg·L of NP was inhibited at 125 dpf in the F1 generation. 20 μg·L of NP resulted in 80% females in the F1 generation, but had no effect on the F2 generation. In terms of the sexual differentiation genes, the transcriptional expression of cyp19a1a and esr1 genes were upregulated in 20 μg·L of NP in the F1 generation. But expression of the sexual differentiation genes were not affected in the F2 generation. Overall, NP could affect sexual differentiation and gene transcriptional expression in the F1 generation. The tolerance of contaminant in the offsprings was improved at low concentration.
壬基酚（NP）是一种持久性环境化学物质，可破坏生物体的内分泌系统，在地表水和海水中都有检出。本研究调查了 NP 是否会改变性别分化相关基因的转录表达。将三代斑马鱼暴露于 0、2、20 和 200μg·L 的 NP 中，并在 F1 和 F2 代的 10、20 和 40 dpf 时评估性别分化基因的转录表达。在 F1 代中，暴露于 200μg·L NP 的斑马鱼在 125 dpf 时生长受到抑制。20μg·L 的 NP 导致 F1 代中 80%的雌性，但对 F2 代没有影响。就性别分化基因而言，cyp19a1a 和 esr1 基因在 F1 代中的 20μg·L NP 中表达上调。但在 F2 代中，性别分化基因的表达没有受到影响。总体而言，NP 可能会影响 F1 代的性别分化和基因转录表达。在低浓度下，后代对污染物的耐受性得到提高。
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