Research Center of Hydrobiology, Department of Ecology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510632, China.
School of Life Science and Engineering, State Defense Key Laboratory of the Nuclear Waste and Environmental Security, Southwest University of Science and Technology, Mianyang 621010, China.
Int J Environ Res Public Health. 2020 Feb 4;17(3):970. doi: 10.3390/ijerph17030970.
Benzo[a]pyrene (BaP) is a common environmental disrupting chemical that can cause endocrine disorders in organisms. However, the continued interference effects of BaP on multi-generation fish needs further research. In this study, we performed different periods (G1F1-3, G2F2-3, G3F3) of BaP exposure on marine medaka. We determined the embryo toxicity, and analyzed relative reproductive genes (ERα, cyp19a and vtg1) to predict the sexual differentiation of marine medaka. The results showed that high concentrations of BaP (200 μg·L) significantly delayed the hatching time of embryos. Moreover, medium/high concentrations of BaP (20 and 200 μg·L) prolonged the sexual maturity time of marine medaka. The relative gene expression of ERα, cyp19a and vtg1 were measured at 5 d of embryos. We found that BaP had significantly inhibited the expression of the genes related to female fish development. Consequently, there were more males in the offspring sex ratio at BaP exposure. Overall, BaP can cause embryonic toxicity and abnormal sexual differentiation, while the expression of related reproductive genes can effectively indicate the sex ratio.
苯并[a]芘（BaP）是一种常见的环境扰乱化学物质，可导致生物体内分泌失调。然而，BaP 对多代鱼类的持续干扰效应仍需要进一步研究。本研究通过对海水斑马鱼进行不同时期（G1F1-3、G2F2-3、G3F3）的 BaP 暴露，测定胚胎毒性，并分析相对生殖基因（ERα、cyp19a 和 vtg1），以预测海水斑马鱼的性别分化。结果表明，高浓度 BaP（200μg·L）显著延迟了胚胎的孵化时间。此外，中/高浓度 BaP（20 和 200μg·L）延长了海水斑马鱼的性成熟时间。在胚胎第 5 天测量 ERα、cyp19a 和 vtg1 的相对基因表达。我们发现 BaP 显著抑制了与雌性鱼类发育相关基因的表达。因此，BaP 暴露组的后代性别比例中雄性更多。总的来说，BaP 可导致胚胎毒性和异常的性别分化，而相关生殖基因的表达可有效指示性别比例。
