一个表达恶性疟原虫棒状体蛋白的cDNA克隆。

A cDNA clone expressing a rhoptry protein of Plasmodium falciparum.

作者信息

Coppel R L, Bianco A E, Culvenor J G, Crewther P E, Brown G V, Anders R F, Kemp D J

机构信息

Walter and Eliza Hall Institute of Medical Research, Victoria, Australia.

出版信息

Mol Biochem Parasitol. 1987 Aug;25(1):73-81. doi: 10.1016/0166-6851(87)90020-x.

DOI:10.1016/0166-6851(87)90020-x

PMID:2823136

Abstract

Antibodies from immune humans were used to select a cDNA clone expressing an asexual blood stage antigen of Plasmodium falciparum. The expressed fused polypeptide was used as an affinity reagent to purify human antibodies specific for the corresponding parasite antigen. Western blotting and immunoelectronmicroscopy demonstrated that the antigen was a 105 kDa protein located in the rhoptries of merozoites. The cDNA encodes the carboxy terminus of the rhoptry antigen, a sequence rich both in charged and hydroxy amino acids.

摘要

利用免疫人群的抗体筛选出一个表达恶性疟原虫无性血液期抗原的cDNA克隆。将表达的融合多肽用作亲和试剂，以纯化针对相应寄生虫抗原的人源抗体。蛋白质免疫印迹法和免疫电子显微镜检查表明，该抗原是一种位于裂殖子棒状体中的105 kDa蛋白质。该cDNA编码棒状体抗原的羧基末端，这是一个富含带电荷和羟基氨基酸的序列。