Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Int J Biochem Cell Biol. 2017 Apr;85:135-148. doi: 10.1016/j.biocel.2017.01.016. Epub 2017 Feb 22.
The present research was conducted to evaluate the effect of bone marrow derived mesenchymal stem cells (BM-MSCs) as a potential therapeutic tool for improvement of skeletal muscle recovery after induced chemodenervation atrophy by repeated local injection of botulinum toxin-A in the right tibialis anterior muscle of adult male albino rats. Forty five adult Wistar male albino rats were classified into control and experimental groups. Experimental group was further subdivided into 3 equal subgroups; induced atrophy, BM-MSCs treated and recovery groups. Biochemical analysis of serum LDH, CK and Real-time PCR for Bcl-2, caspase 3 and caspase 9 was measured. Skeletal muscle sections were stained with H and E, Mallory trichrome, and Immunohistochemical reaction for Bax and CD34. Improvement in the skeletal muscle histological structure was noticed in BM-MSCs treated group, however, in the recovery group, some sections showed apparent transverse striations and others still affected. Immunohistochemical reaction of Bax protein showed strong positive immunoreaction in the cytoplasm of muscle fibers in the induced atrophy group. BM-MSCs treated group showed weak positive reaction while the recovery group showed moderate reaction in the cytoplasm of muscle fibers. Immunohistochemical reaction for CD34 revealed occasional positive CD34 stained cells in the induced atrophy group. In BM-MSCs treated group, multiple positive CD34 stained cells were detected. However, recovery group showed some positive CD34 stained cells at the periphery of the muscle fibers. Marked improvement in the regenerative capacity of skeletal muscles after BM-MSCs therapy. Hence, stem cell therapy provides a new hope for patients suffering from myopathies and severe injuries.
本研究旨在评估骨髓间充质干细胞（BM-MSCs）作为一种潜在的治疗工具，通过在成年雄性白化大鼠右侧胫骨前肌中重复局部注射肉毒毒素-A 来改善化学去神经萎缩后骨骼肌的恢复。45 只成年 Wistar 雄性白化大鼠被分为对照组和实验组。实验组进一步分为 3 个相等的亚组；诱导萎缩、BM-MSCs 治疗和恢复组。测量血清 LDH、CK 的生化分析和 Bcl-2、caspase 3 和 caspase 9 的实时 PCR。对骨骼肌切片进行 H 和 E、Mallory 三色和 Bax 和 CD34 的免疫组织化学反应染色。BM-MSCs 治疗组骨骼肌组织学结构得到改善，然而，在恢复组，一些切片仍显示明显的横纹，而另一些切片仍受到影响。Bax 蛋白的免疫组织化学反应显示，在诱导萎缩组的肌纤维细胞质中存在强烈的阳性免疫反应。BM-MSCs 治疗组显示弱阳性反应，而恢复组显示肌纤维细胞质中的中等反应。CD34 的免疫组织化学反应显示，在诱导萎缩组偶尔有阳性 CD34 染色细胞。在 BM-MSCs 治疗组中，检测到多个阳性 CD34 染色细胞。然而，恢复组在肌纤维的外围显示出一些阳性 CD34 染色细胞。BM-MSCs 治疗后骨骼肌再生能力明显改善。因此，干细胞治疗为患有肌肉疾病和严重损伤的患者提供了新的希望。
