Artioli Guilherme Giannini, De Oliveira Silvestre João Guilherme, Guilherme João Paulo Limongi França, Baptista Igor Luchini, Ramos Gracielle Vieira, Da Silva Willian José, Miyabara Elen Haruka, Moriscot Anselmo Sigari
Laboratory of Cellular and Molecular Biology of Striated Muscle, Department of Anatomy, Institute of Biomedical Sciences, Avenida Prof. Lineu Prestes 2415, São Paulo CEP 05508-000, Brazil; Laboratory of Applied Nutrition and Metabolism, School of Physical Education and Sport, Department of Biodynamics, University of São Paulo, São Paulo, SP, Brazil.
Muscle Nerve. 2015 Mar;51(3):346-52. doi: 10.1002/mus.24320. Epub 2015 Jan 7.
We injected embryonic stem cells into mouse tibialis anterior muscles subjected to botulinum toxin injections as a model for reversible neurogenic atrophy.
Muscles were exposed to botulinum toxin for 4 weeks and allowed to recover for up to 6 weeks. At the onset of recovery, a single muscle injection of embryonic stem cells was administered. The myofiber cross-sectional area, single twitch force, peak tetanic force, time-to-peak force, and half-relaxation time were determined.
Although the stem cell injection did not affect the myofiber cross-sectional area gain in recovering muscles, most functional parameters improved significantly compared with those of recovering muscles that did not receive the stem cell injection.
Muscle function recovery was accelerated by embryonic stem cell delivery in this durable neurogenic atrophy model. We conclude that stem cells should be considered a potential therapeutic tool for recovery after extreme skeletal muscle atrophy.
我们将胚胎干细胞注射到接受肉毒杆菌毒素注射的小鼠胫前肌中，以此作为可逆性神经源性萎缩的模型。
肌肉暴露于肉毒杆菌毒素4周，并允许恢复长达6周。在恢复开始时，对肌肉进行单次胚胎干细胞注射。测定肌纤维横截面积、单收缩力、强直收缩峰值力、达到峰值力的时间和半松弛时间。
尽管干细胞注射并未影响恢复中肌肉的肌纤维横截面积增加，但与未接受干细胞注射的恢复中肌肉相比，大多数功能参数均有显著改善。
在这个持续性神经源性萎缩模型中，胚胎干细胞递送加速了肌肉功能恢复。我们得出结论，干细胞应被视为极端骨骼肌萎缩后恢复的潜在治疗工具。
