Liu Qiu-Ning, Xin Zhao-Zhe, Liu Yu, Wang Zheng-Fei, Chen Yi-Jing, Zhang Dai-Zhen, Jiang Sen-Hao, Chai Xin-Yue, Zhou Chun-Lin, Tang Bo-Ping
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng 224001, Jiangsu Province, People's Republic of China.
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng 224001, Jiangsu Province, People's Republic of China.
Fish Shellfish Immunol. 2017 Apr;63:297-303. doi: 10.1016/j.fsi.2017.02.025. Epub 2017 Feb 21.
Ferritin plays important roles in iron storage, detoxification, and immune response. Here, a ferritin gene (PcFer) was identified in Procambarus clarkii, an economically important freshwater crayfish. Full-length PcFer cDNA was 1022-bp, including a 135-bp 5'-untranslated region (UTR) with a typical iron responsive element, a 374-bp 3'-UTR, and a 513-bp open reading frame encoding a polypeptide of 170 amino acids which contained the Ferritin domain. PcFer has ion binding sites, a ferrihydrite nucleation center, and an iron ion channel. PcFer is phylogenetically closely-related to Pacifastacus leniusculus and Eriocheir sinensis ferritins. Real-time quantitative reverse-transcription PCR analysis showed that PcFer was expressed in all tested P. clarkii tissues, and expressed most in hepatopancreas. After challenge with various heavy metals and lipopolysaccharide, respectively, the hepatopancreatic expression levels of PcFer were markedly upregulated. These results suggest that expression of PcFer might be involved in immune defense and protection of P. clarkii against heavy metal stress.
铁蛋白在铁储存、解毒和免疫反应中发挥着重要作用。在此，在经济上重要的淡水小龙虾克氏原螯虾中鉴定出一个铁蛋白基因（PcFer）。PcFer cDNA全长1022bp，包括一个135bp的5'-非翻译区（UTR），带有一个典型的铁反应元件，一个374bp的3'-UTR，以及一个513bp的开放阅读框，编码一个含有170个氨基酸的多肽，该多肽包含铁蛋白结构域。PcFer具有离子结合位点、水铁矿成核中心和铁离子通道。PcFer在系统发育上与美洲鳌龙虾和中华绒螯蟹的铁蛋白密切相关。实时定量逆转录PCR分析表明，PcFer在所有测试的克氏原螯虾组织中均有表达，在肝胰腺中表达最多。分别用各种重金属和脂多糖刺激后，PcFer在肝胰腺中的表达水平明显上调。这些结果表明，PcFer的表达可能参与克氏原螯虾的免疫防御以及对重金属胁迫的保护作用。
