Minenko A, Kiselev G, Tulkova E, Oehme P
Institute of Drug Research, Academy of Sciences of the GDR, Berlin.
Pharmazie. 1987 May;42(5):341-4.
When rat adrenal medulla slices, treated with atropine (1 X 10(-6) mol X l-1], were stimulated with acetylcholine (1 X 10(-5) mol X l-1), an enhanced 32P incorporation into phosphatidylinositol-bis-phosphate (PIP2) and phosphatidylphosphate (PIP) was observed. On the ather hand, stimulation of myo-[3H] inositol prelabelled slices led to a decline in radioactivity of PIP2, indicating an increased turnover of this phospholipid. A possible affection of monoesterphosphate groups of PIP2 or of both PIP2 and PIP during cholinergic nicotinic stimulation is assumed.
当用阿托品（1×10⁻⁶摩尔/升）处理的大鼠肾上腺髓质切片用乙酰胆碱（1×10⁻⁵摩尔/升）刺激时，观察到磷脂酰肌醇二磷酸（PIP2）和磷脂酰磷酸（PIP）中³²P掺入增加。另一方面，对预先用肌醇-[³H]标记的切片进行刺激导致PIP2放射性下降，表明这种磷脂的周转率增加。推测在胆碱能烟碱样刺激过程中PIP2的单酯磷酸基团或PIP2和PIP两者可能受到影响。
