Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011, USA.
Department of Agronomy, Iowa State University, Ames, Iowa 50011, USA.
Nat Commun. 2017 Feb 24;8:14573. doi: 10.1038/ncomms14573.
Brassinosteroids (BRs) regulate plant growth and stress responses via the BES1/BZR1 family of transcription factors, which regulate the expression of thousands of downstream genes. BRs are involved in the response to drought, however the mechanistic understanding of interactions between BR signalling and drought response remains to be established. Here we show that transcription factor RD26 mediates crosstalk between drought and BR signalling. When overexpressed, BES1 target gene RD26 can inhibit BR-regulated growth. Global gene expression studies suggest that RD26 can act antagonistically to BR to regulate the expression of a subset of BES1-regulated genes, thereby inhibiting BR function. We show that RD26 can interact with BES1 protein and antagonize BES1 transcriptional activity on BR-regulated genes and that BR signalling can also repress expression of RD26 and its homologues and inhibit drought responses. Our results thus reveal a mechanism coordinating plant growth and drought tolerance.
油菜素内酯（BRs）通过 BES1/BZR1 转录因子家族调节植物的生长和应激反应，该家族调节数千个下游基因的表达。BRs 参与对干旱的响应，然而，BR 信号与干旱响应之间相互作用的机制仍有待确定。在这里，我们表明转录因子 RD26 介导了干旱和 BR 信号之间的串扰。当过度表达时，BES1 靶基因 RD26 可以抑制 BR 调节的生长。全基因表达研究表明，RD26 可以拮抗 BR 调节一组 BES1 调节基因的表达，从而抑制 BR 功能。我们表明，RD26 可以与 BES1 蛋白相互作用并拮抗 BES1 在 BR 调节基因上的转录活性，并且 BR 信号也可以抑制 RD26 及其同源物的表达并抑制干旱反应。因此，我们的研究结果揭示了一种协调植物生长和耐旱性的机制。
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