Xu Shirui, Wei Zihao, Ma Mingchuan, Zhang Lijun, Liu Zhang, Liu Longlong
Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan, Shanxi, China.
Germplasm Enhancement on Loess Plateau, Ministry of Agriculture and Rural Affairs, Taiyuan, Shanxi, China.
Front Plant Sci. 2025 Aug 14;16:1616026. doi: 10.3389/fpls.2025.1616026. eCollection 2025.
The Brassinazole-resistant (BZR) family of transcription factors acts as key regulators in brassinosteroid (BR) signaling, influencing plant growth, development, biotic and abiotic stresses. However, systematic analysis of the genes in oat has not been conducted. Moreover, little is known about their functions in osmotic stress, which is a major abiotic stress affecting oat production.
In this study, we performed a genome-wide analysis of the gene family in oat. Their chromosome locations, gene structures, phylogenetic relationships, conserved domains, promoter cis-elements, and gene duplication events were analyzed. Furthermore, the expression patterns of genes under osmotic stress were characterized, and the subcellular localization of AsBZR12 was investigated in .
In this study, we mapped 14 members of the gene family across 12 oat chromosomes, and classified them into three groups based on phylogenetic analysis. The BZR proteins displayed group-specific patterns in their exon-intron structures and conserved motifs. Furthermore, cis-acting element analysis revealed that genes are primarily involved in phytohormone responses and environmental stress adaptation. Examination of gene duplication revealed that segmental duplications drove the expansion of the gene family in the oat genome, with evidence of strong purifying selection pressure during evolutionary development. The qRT-PCR analysis demonstrated varied expression patterns among members. Specifically, was significantly upregulated in roots, stems, and leaves, with nuclear localization. In summary, our study provides a comprehensive analysis of the genes and characterizes their expression patterns under osmotic stress conditions, thereby identifying potential candidate genes for future research. This research provides comprehensive insights into gene structure and evolution, establishing a foundation for understanding their osmotic stress responses in oat.
抗油菜素唑（BZR）转录因子家族在油菜素类固醇（BR）信号传导中起关键调节作用，影响植物生长、发育、生物和非生物胁迫。然而，尚未对燕麦中的这些基因进行系统分析。此外，关于它们在渗透胁迫中的功能知之甚少，而渗透胁迫是影响燕麦产量的主要非生物胁迫。
在本研究中，我们对燕麦中的该基因家族进行了全基因组分析。分析了它们的染色体定位、基因结构、系统发育关系、保守结构域、启动子顺式元件和基因复制事件。此外，还对渗透胁迫下该基因的表达模式进行了表征，并在……中研究了AsBZR12的亚细胞定位。
在本研究中，我们将该基因家族的14个成员定位到12条燕麦染色体上，并根据系统发育分析将它们分为三组。BZR蛋白在其外显子 - 内含子结构和保守基序中表现出组特异性模式。此外，顺式作用元件分析表明，该基因主要参与植物激素反应和环境胁迫适应。基因复制检查表明，片段重复推动了该基因家族在燕麦基因组中的扩展，在进化发育过程中有强烈的纯化选择压力的证据。qRT-PCR分析表明该家族成员之间的表达模式各不相同。具体而言，……在根、茎和叶中显著上调，具有核定位。总之，我们的研究对该基因进行了全面分析，并表征了它们在渗透胁迫条件下的表达模式，从而为未来研究确定了潜在的候选基因。这项研究为该基因的结构和进化提供了全面的见解，为理解它们在燕麦中的渗透胁迫反应奠定了基础。
