[在绿猴肾细胞培养物中分离出人类轮状病毒]
[Isolation of the human rotavirus in a cell culture of green monkey kidneys].
作者信息
Vasil'eva V I, Gracheva N M, Karazhas N V, Blokhina T A, Avakov A A
出版信息
Vopr Virusol. 1987 May-Jun;32(3):360-2.
Properties of a virus isolated from a patient with rotavirus gastroenteritis (the diagnosis verified by direct electron microscopy) were studied. The virus was identified as a rotavirus in tissue culture neutralization and complement fixation tests. It was shown to replicate through a significant number of passages in continuous cell cultures (Vero, RH) as well as in primary cell cultures of human and animal origin (human embryo kidney, green monkey kidney); some features associated with rotavirus propagation are described.
对从一名轮状病毒肠胃炎患者（通过直接电子显微镜检查确诊）分离出的一种病毒的特性进行了研究。在组织培养中和试验及补体结合试验中，该病毒被鉴定为轮状病毒。结果表明，它能在连续细胞培养物（Vero细胞、RH细胞）以及人和动物来源的原代细胞培养物（人胚肾细胞、绿猴肾细胞）中经大量传代进行复制；文中描述了一些与轮状病毒增殖相关的特征。
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