热疗通过早期分化过程中长链非编码核糖核酸（lncRNAs）的改变影响神经干细胞的命运决定。
Hyperthermia influences fate determination of neural stem cells with lncRNAs alterations in the early differentiation.
作者信息
Wang Lei, Deng Yujia, Duan Da, Sun Shuaiqi, Ge Lite, Zhuo Yi, Yuan Ting, Wu Pei, Wang Hao, Lu Ming, Xia Ying
机构信息
Department of Neurosurgery, Affiliated Haikou Hospital, Xiangya School of Central South University, Haikou, Hsinan, China.
Department of Neurosurgery, the Second Affiliated Hospital of Hunan Normal University (PLA 163 Hospital), Changsha, Hunan, China.
出版信息
PLoS One. 2017 Feb 24;12(2):e0171359. doi: 10.1371/journal.pone.0171359. eCollection 2017.
BACKGROUND
Temperature is an important parameter in the microenvironment of neural stem cells (NSCs); however, little is known about the precise effects of hyperthermia on fate determination in NSCs or the role of long non-coding (lnc)RNAs in this process. This was addressed in the present study using NSCs cultured at two different temperatures.
METHODS
NSCs were divided into 37NSC and 40NSC groups that were cultured at 37°C or 40°C, respectively, for 72 h. Neuronal or glial cell differentiation was evaluated by flow cytometry and western blotting. LncRNA expression was detected by quantitative real-time PCR.
RESULTS
The numbers of cells positive for the neuronal marker Tuj-1 and the glial cell marker glial fibrillary acidic protein were higher in the 40NSC than in the 37NSC group. The two groups also showed distinct lncRNA expression profiles.
CONCLUSION
Hyperthermia promotes neuronal and glial differentiation in NSCs, which involves specific lncRNAs.
背景
温度是神经干细胞（NSCs）微环境中的一个重要参数；然而，关于热疗对神经干细胞命运决定的确切影响或长链非编码（lnc）RNA在此过程中的作用，我们知之甚少。本研究通过在两种不同温度下培养神经干细胞来解决这一问题。
方法
将神经干细胞分为37NSC组和40NSC组，分别在37°C或40°C下培养72小时。通过流式细胞术和蛋白质免疫印迹法评估神经元或神经胶质细胞的分化。通过定量实时PCR检测lncRNA表达。
结果
40NSC组中神经元标志物Tuj-1和神经胶质细胞标志物胶质纤维酸性蛋白阳性的细胞数量高于37NSC组。两组还显示出不同的lncRNA表达谱。
结论
热疗促进神经干细胞向神经元和神经胶质细胞分化，这涉及特定的lncRNA。
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