Isolation of high-molecular-weight DNA from eukaryotic cells by formamide treatment and dialysis.
作者信息
Kupiec J J, Giron M L, Vilette D, Jeltsch J M, Emanoil-Ravier R
机构信息
UER d'Hématologie, LOI CNRS, Hôpital Saint-Louis, Paris, France.
出版信息
Anal Biochem. 1987 Jul;164(1):53-9. doi: 10.1016/0003-2697(87)90366-6.
PMID:2823634
Abstract
A new method for the isolation of high-molecular-weight DNA from eukaryotic cells is presented. It is based on formamide treatment and extensive dialysis of cellular proteinase K digests. This procedure consists mainly of a series of incubations allowing simultaneous preparation of several DNAs which can then be restricted, ligated, hybridized, and cloned.
摘要
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