Department of Pharmacy, Huzhou University, Huzhou 313000, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
Chin J Nat Med. 2016 Oct;14(10):783-788. doi: 10.1016/S1875-5364(16)30093-0. Epub 2016 Oct 31.
The present study was designed to construct the structurally diverse library of tetrahydroprotoberberines (THPBs) by combining the methods of chemical nonselective demethylation and microbial glycosylation. HPLC-MS/MS analyses tentatively identified 12 de-methylated and 9 glycosylated derivates of THPBs and 5 rarely oxidized glycosides of THPBs in the library. Through this effort, we achieved not only a variety of the THPBs and their glycosides but also tested the catalytic characteristics and capabilities of G. deliquescens NRRL 1086.
本研究旨在通过结合化学非选择性脱甲基化和微生物糖基化方法，构建结构多样的四氢原小檗碱（THPBs）文库。HPLC-MS/MS 分析初步鉴定出文库中 12 个脱甲基化和 9 个糖基化的 THPBs 衍生物以及 5 个 THPBs 的罕见氧化糖基化产物。通过这一努力，我们不仅获得了多种 THPBs 及其糖苷，还测试了地衣芽孢杆菌 NRRL 1086 的催化特性和能力。
