Charpentier B, Michelson S, Lantz O, Hiesse C, Martin B, Fries D
Laboratoire de Transplantation d'Organes, Hôpital de Bicêtre, Kremlin-Bicêtre.
Ann Med Interne (Paris). 1987;138(5):334-7.
Cytomegalovirus (CMV)-specific immune T-cells, present in the circulation of previously infected kidney transplant recipients (anti-CMV serum antibody titer greater than 1/40) can be reactivated to give rise to specific helper and cytotoxic effector cells by co-culturation with fresh autologous blasts coated with CMV antigen in vitro. The reactivated cells are able: 1) to proliferate when stimulated with CMV antigen, 2) to kill the autologous target coated with CMV antigen and not autologous blasts alone when assayed in 51Cr release test. These specific effector cells lie in the E+ T cell subset by removing NK cells with sheep-erythrocytes rosetting. When the reactivated cells are tested against allogeneic CMV coated blasts the level of cytotoxicity is in general related to the extent of HLA-A and B antigen sharing between effector and target cells. The results provide strong evidence that: 1) only previously infected individuals can generate immune T cells against CMV in vitro, 2) there is a correlation between the index of proliferation and cytolysis, 3) there is an HLA restriction of immune T cell cytotoxicity of CMV, 4) monoclonal antibodies directed at several antigenic sites of CMV are able to block cytolysis.
巨细胞病毒（CMV）特异性免疫T细胞存在于既往感染过的肾移植受者的循环系统中（抗CMV血清抗体滴度大于1/40），通过与体外包被有CMV抗原的新鲜自体胚细胞共培养，可被重新激活，产生特异性辅助性T细胞和细胞毒性效应细胞。重新激活的细胞能够：1）在受到CMV抗原刺激时增殖；2）在51Cr释放试验中进行检测时，能够杀伤包被有CMV抗原的自体靶细胞，而不仅仅是单独的自体胚细胞。通过用绵羊红细胞花环法去除自然杀伤细胞（NK细胞），这些特异性效应细胞属于E + T细胞亚群。当针对同种异体CMV包被的胚细胞检测重新激活的细胞时，细胞毒性水平通常与效应细胞和靶细胞之间HLA - A和B抗原的共享程度相关。这些结果提供了强有力的证据表明：1）只有既往感染过的个体才能在体外产生针对CMV的免疫T细胞；2）增殖指数与细胞溶解之间存在相关性；3）CMV免疫T细胞的细胞毒性存在HLA限制；4）针对CMV多个抗原位点的单克隆抗体能够阻断细胞溶解。
