Villacres M C, Lacey S F, La Rosa C, Krishnan R, Auge C, Longmate J, Zaia J A, Leedom J M, Diamond D J
Laboratory of Vaccine Research, Department of Virology, Beckman Research Institute, City of Hope National Medical Center, Duarte, California, USA.
J Infect Dis. 2001 Aug 1;184(3):256-67. doi: 10.1086/322028. Epub 2001 Jul 10.
CD8(+) T lymphocyte function specific for human cytomegalovirus (CMV) was evaluated in 14 patients infected with human immunodeficiency virus (HIV) receiving highly active antiretroviral therapy (HAART) and 26 CMV-seropositive donors without HIV infection. Fifty-seven percent of the HIV-infected group had CMV-specific cytolytic activity in freshly isolated peripheral blood mononuclear cells (PBMC) against targets expressing CMV pp65. Both interferon (IFN)-gamma secretion by CD8(+) T cells and the frequency of human leukocyte antigen (HLA)-tetramer-positive T cells in HLA-A*0201-positive HIV-infected subjects correlated with CMV-specific cytolysis. In contrast, PBMC from healthy CMV-seropositive donors did not have either measurable CMV-specific cytolysis or secretion of IFN-gamma without in vitro stimulation. The T helper response to CMV antigens was vigorous in healthy CMV-seropositive donors but low in the cohort of HIV-infected patients. Potent CD8(+) cytotoxic T lymphocyte responses to CMV in HIV-infected patients receiving HAART is the converse of what is found in healthy CMV-seropositive subjects and may be the predominant adaptive immune response against CMV in HIV-infected patients.
在14名接受高效抗逆转录病毒治疗（HAART）的人类免疫缺陷病毒（HIV）感染者和26名未感染HIV的巨细胞病毒（CMV）血清反应阳性供者中，评估了针对人巨细胞病毒的CD8(+) T淋巴细胞功能。在HIV感染组中，57%的患者新鲜分离的外周血单核细胞（PBMC）对表达CMV pp65的靶细胞具有CMV特异性细胞溶解活性。在HLA-A*0201阳性的HIV感染者中，CD8(+) T细胞分泌的干扰素（IFN）-γ以及人白细胞抗原（HLA）-四聚体阳性T细胞的频率与CMV特异性细胞溶解相关。相比之下，健康的CMV血清反应阳性供者的PBMC在没有体外刺激的情况下，既没有可测量的CMV特异性细胞溶解，也没有IFN-γ分泌。健康的CMV血清反应阳性供者对CMV抗原的T辅助反应强烈，但在HIV感染患者队列中则较低。接受HAART的HIV感染患者对CMV有强大的CD8(+) 细胞毒性T淋巴细胞反应，这与健康的CMV血清反应阳性受试者的情况相反，可能是HIV感染患者针对CMV的主要适应性免疫反应。
