Ansari Mahdi, Zhandi Mahdi, Kohram Hamid, Zaghari Mojtaba, Sadeghi Mostafa, Sharafi Mohsen
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran.
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran.
Theriogenology. 2017 Apr 1;92:69-74. doi: 10.1016/j.theriogenology.2017.01.014. Epub 2017 Jan 8.
This study was conducted to investigate the effect of d-Aspartic acid (D-Asp) on post-thawed sperm quality, fertility and hatchability outcomes in male broiler breeders. Twenty 55-week-old roosters were selected and equally split into four groups (n = 5 rooster/group). Different daily D-Asp doses including 0 (D-0), 100 (D-100), 200 (D-200) or 300 (D-300) mg/kg BW were capsulated and individually administered for 12 weeks to roosters in each group. Semen samples were weekly collected from 7th to 12th week of experiment. Sperm quality from 7th to 11th week was evaluated in both fresh (total and forward motility and plasma membrane functionality) and post-thawed (total and forward motility, plasma membrane functionality, apoptosis status and mitochondrial activity) conditions. Also, collected semen samples on the 12th week were frozen and artificially inseminated to evaluate fertility and hatchability. The results from fresh condition showed that total and forward motility and plasma membrane functionality were significantly higher in D-200 compared to other groups. Also, interaction effect of time and treatment was not significant for all assessed parameters in fresh condition. In post-thawed condition, D-200 showed significantly higher total and forward motility, fertility and hatchability compared to other groups. The higher value for plasma membrane functionality and mitochondrial activity was observed in D-200 compared to D-0 and D300 groups. However, the percentage of live, early apoptotic and dead spermatozoa were not significantly affected by applied treatment in the current study. No significant difference for time and treat interaction effect was observed for all assessed parameters except forward motility. In conclusion, it seems that D-Asp administration could improve fresh and post-thawed sperm quality and post-thawed sperm fertility in male broiler breeders.
本研究旨在调查d-天冬氨酸（D-Asp）对雄性肉种鸡解冻后精子质量、受精率和孵化率的影响。选取20只55周龄的公鸡，平均分为四组（每组n = 5只公鸡）。将不同的每日D-Asp剂量（包括0（D-0）、100（D-100）、200（D-200）或300（D-300）mg/kg体重）制成胶囊，分别给每组公鸡单独服用12周。在实验的第7至12周每周采集精液样本。对第7至11周的精子质量在新鲜状态（总活力和前向运动能力以及质膜功能）和解冻后状态（总活力和前向运动能力、质膜功能、凋亡状态和线粒体活性）下进行评估。此外，将第12周采集的精液样本冷冻并进行人工授精，以评估受精率和孵化率。新鲜状态下的结果表明，与其他组相比，D-200组的总活力、前向运动能力和质膜功能显著更高。此外，在新鲜状态下，时间和处理的交互作用对所有评估参数均无显著影响。在解冻后状态下，与其他组相比，D-200组的总活力、前向运动能力、受精率和孵化率显著更高。与D-0组和D300组相比，D-200组的质膜功能和线粒体活性值更高。然而，在本研究中，所应用的处理对活精子、早期凋亡精子和死精子的百分比没有显著影响。除前向运动能力外，所有评估参数在时间和处理交互作用方面均未观察到显著差异。总之，似乎给予D-Asp可以提高雄性肉种鸡新鲜和解冻后精子的质量以及解冻后精子的受精率。
