Gobikrushanth M, Dutra P A, Bruinjé T C, Colazo M G, Butler S T, Ambrose D J
Department of Agricultural Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
Livestock Research and Extension Branch, Alberta Agriculture and Forestry, Edmonton, AB T6H 5T6, Canada.
Theriogenology. 2017 Apr 1;92:90-94. doi: 10.1016/j.theriogenology.2017.01.018. Epub 2017 Jan 11.
The objectives were to determine (1) the repeatability of antral follicle counts (AFC) and anti-Müllerian hormone (AMH) concentrations at an unknown stage of follicular growth (FG) and at an expected day of follicular wave emergence (FWE) in dairy cows, and (2) the association between AFC and AMH at the above two stages. Transrectal ultrasound imaging of the ovaries and blood sample collection were performed on 91 lactating Holstein cows (33 primiparous, 58 multiparous) to determine AFC and serum concentrations of AMH at an unknown stage of FG (mean ± SEM; 14 ± 0.5 d postpartum) and an expected day of FWE (mean ± SEM; 73 ± 0.5 d postpartum, approximately 36 h after the 2nd GnRH of Ovsynch protocol). The AFC ranged from 10 to 53 (mean ± SEM; 26.1 ± 1.0) and 6 to 45 (mean ± SEM; 23.4 ± 1.0) at an unknown stage of FG and expected day of FWE, respectively (P = 0.02). Serum concentrations (pg/mL) of AMH ranged from 13.9 to 528.8 (mean ± SEM; 187.3 ± 13.1) and 38.2 to 774.1 (mean ± SEM; 218.7 ± 14.5) at an unknown stage of FG and expected day of FWE, respectively (P < 0.01). The repeatability estimates for AFC and serum AMH concentrations between an unknown stage of FG and the expected day of FWE were 0.37 and 0.73 (P < 0.01), respectively. The correlation between AFC and AMH was moderate at an unknown stage of FG (r = 0.54; P < 0.01) and at an expected day of FWE (r = 0.59; P < 0.01). In summary, both AFC and AMH were repeatable when measured at an unknown stage of FG and an expected day of FWE, but the repeatability was greater for AMH. Thus, if cows are tested at an unknown stage of FG, serum AMH may be a more reliable phenotype than AFC to test association with reproductive outcomes. Furthermore, AFC and AMH were moderately correlated at the two stages examined.
(1) 在奶牛卵泡生长（FG）的未知阶段以及卵泡波出现（FWE）的预期日，窦状卵泡计数（AFC）和抗苗勒管激素（AMH）浓度的重复性；(2) 在上述两个阶段AFC与AMH之间的关联。对91头泌乳期荷斯坦奶牛（33头初产牛，58头经产牛）进行经直肠卵巢超声成像和血样采集，以测定FG未知阶段（平均±标准误；产后14±0.5天）和FWE预期日（平均±标准误；产后73±0.5天，即Ovsynch方案第二次促性腺激素释放激素（GnRH）注射后约36小时）的AFC和血清AMH浓度。在FG未知阶段和FWE预期日，AFC分别为10至53（平均±标准误；26.1±1.0）和6至45（平均±标准误；23.4±1.0）（P = 0.02）。在FG未知阶段和FWE预期日，血清AMH浓度（pg/mL）分别为13.9至528.8（平均±标准误；187.3±13.1）和38.2至774.1（平均±标准误；218.7±14.5）（P < 0.01）。FG未知阶段与FWE预期日之间AFC和血清AMH浓度的重复性估计值分别为0.37和0.73（P < 0.01）。在FG未知阶段（r = 0.54；P < 0.01）和FWE预期日（r = 0.59；P < 0.01），AFC与AMH之间的相关性为中等。总之，在FG未知阶段和FWE预期日测量时，AFC和AMH均具有重复性，但AMH的重复性更高。因此，如果在FG未知阶段对奶牛进行检测，血清AMH可能是比AFC更可靠的表型，用于检测与繁殖结果的关联。此外，在所检测的两个阶段，AFC与AMH呈中等程度相关。
