“无神经突”N1A - 103小鼠神经母细胞瘤细胞系中RI cAMP结合蛋白的诱导

Induction of the RI cAMP-binding protein in the "neurite-minus" N1A-103 mouse neuroblastoma cell line.

作者信息

Liu A Y, Kamalakannan V, Lin Z

机构信息

Department of Biological Sciences, Rutgers, University, Piscataway, N.J. 08855.

出版信息

Biochem Biophys Res Commun. 1987 Oct 14;148(1):350-6. doi: 10.1016/0006-291x(87)91117-x.

DOI:10.1016/0006-291x(87)91117-x

PMID:2823808

Abstract

We examined the effects of agents/conditions that raise intracellular cAMP concentration in the induction of the 47,000-dalton RI cAMP-binding protein in the "neurite-minus" N1A-103 mouse neuroblastoma cells. The amount of RI in cell extracts was quantitated by photoaffinity labeling with 8-N3-[32P]cAMP and by ELISA and Western blot. Our results showed that treatment of the N1A-103 neuroblastoma cells with 20 microM forskolin or 1 mM dibutyryl cAMP, or reducing the serum concentration in the culture medium from 10% to 1% caused a 3-4 fold increase in the amount of RJ. Our results suggest that the induction of RJ can occur independent of morphological differentiation in the mouse neuroblastoma cells.

摘要

我们研究了提高细胞内cAMP浓度的试剂/条件对“无神经突”N1A - 103小鼠神经母细胞瘤细胞中47,000道尔顿RI cAMP结合蛋白诱导的影响。通过用8 - N3 - [32P]cAMP进行光亲和标记以及酶联免疫吸附测定（ELISA）和蛋白质免疫印迹法（Western blot）对细胞提取物中的RI量进行定量。我们的结果表明，用20微摩尔佛司可林或1毫摩尔二丁酰环磷腺苷（dibutyryl cAMP）处理N1A - 103神经母细胞瘤细胞，或将培养基中的血清浓度从10%降低到1%，会使RJ的量增加3至4倍。我们的结果表明，在小鼠神经母细胞瘤细胞中，RJ的诱导可以独立于形态分化而发生。