通过基因共靶向与筛选在诱导多能干细胞中实现高效精准基因组编辑
Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection.
作者信息
Mitzelfelt Katie A, McDermott-Roe Chris, Grzybowski Michael N, Marquez Maribel, Kuo Chieh-Ti, Riedel Michael, Lai Shuping, Choi Melinda J, Kolander Kurt D, Helbling Daniel, Dimmock David P, Battle Michele A, Jou Chuanchau J, Tristani-Firouzi Martin, Verbsky James W, Benjamin Ivor J, Geurts Aron M
机构信息
Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA.
Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
出版信息
Stem Cell Reports. 2017 Mar 14;8(3):491-499. doi: 10.1016/j.stemcr.2017.01.021. Epub 2017 Feb 24.
Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-mediated editing event at one locus enriches for HDR-mediated edits at additional loci. This strategy, called co-targeting with selection, improves the probability of isolating cells bearing HDR-mediated variants and accelerates the production of disease models.
诱导多能干细胞中的基因组编辑目前受到识别同源定向修复（HDR）修饰细胞的繁琐和昂贵性质的阻碍。我们提出了一种方法，即在一个位点分离携带可选择的、HDR介导的编辑事件的细胞，可富集其他位点的HDR介导的编辑。这种称为选择共靶向的策略提高了分离携带HDR介导变体的细胞的概率，并加速了疾病模型的构建。
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