Jin Gyungsu, Kim Jeongeun, Lee Yunhee, Kim Jinyeong, Akoh Casimir C, Chun Hyang Sook, Ahn Sangdoo, Kim Byung Hee
Department of Chemistry, Chung-Ang University.
J Oleo Sci. 2017 Apr 3;66(4):337-344. doi: 10.5650/jos.ess16154. Epub 2017 Feb 24.
The aim of this study was to discriminate the geographic origin of Korean, Chinese, and Indian sesame oils distributed in Korea using H NMR spectroscopy in combination with canonical discriminant analysis (CDA). H NMR spectra were obtained from 84 roasted oil samples prepared from 51 Korean, 19 Chinese, and 14 Indian sesame seeds. The integration values of 26 peaks observed in the NMR spectra were determined and normalized on the basis of the peak derived from the terminal CH of the fatty acids (0.7446-1.0445 ppm). The variables selected for the CDA include the integration value of one peak (2.7208-2.8533 ppm) that signifies the CH between two C=C bonds from linoleic acid and the integration values of three peaks (2.9811-3.1151 ppm, 3.5914-3.6819 ppm, and 5.9471-5.9625 ppm) attributed to the protons of sesamolin. The CDA results showed that 80 of the 84 oil samples and five of the six additional commercial sesame oil samples were correctly classified based on their production site. This study demonstrated that H NMR spectroscopy is a useful tool to simultaneously analyze the relative abundance of linoleic acid and sesamolin in the sesame oils and good discrimination between the three Asian sesame oils could be achieved when the H NMR analytical data were used in combination with CDA.
本研究的目的是结合典型判别分析（CDA），利用核磁共振氢谱（H NMR）鉴别韩国市场上销售的韩国、中国和印度芝麻油的地理来源。从51份韩国芝麻、19份中国芝麻和14份印度芝麻制备的84份烘焙油样品中获取了H NMR光谱。测定了核磁共振谱中观察到的26个峰的积分值，并以脂肪酸末端CH衍生的峰（0.7446 - 1.0445 ppm）为基础进行归一化。用于CDA的变量包括一个表示亚油酸两个碳碳双键之间CH的峰（2.7208 - 2.8533 ppm）的积分值，以及三个归因于芝麻林素质子的峰（2.9811 - 3.1151 ppm、3.5914 - 3.6819 ppm和5.9471 - 5.9625 ppm）的积分值。CDA结果表明，84份油样中的80份以及另外6份市售芝麻油样品中的5份根据其产地被正确分类。本研究表明，H NMR光谱是同时分析芝麻油中亚油酸和芝麻林素相对丰度的有用工具，当将H NMR分析数据与CDA结合使用时，可以很好地鉴别三种亚洲芝麻油。
