具有游离C末端的肽库的合成与筛选。
Synthesis and screening of peptide libraries with free C-termini.
作者信息
Wang Yen-Chih, Distefano Mark D
机构信息
Departments of Chemistry and Medicinal Chemistry, University of Minnesota, Minneapolis, MN, 55455, USA.
出版信息
Curr Top Pept Protein Res. 2014;15:1-23.
Abstract
Peptide libraries are useful tools to investigate the relationship between structure and function of proteins. The creation of peptide libraries with free C-termini presents unique synthetic challenges. In this review, methods for creating peptide libraries using either solid-phase peptide synthesis or phage display are described. Methods for screening such libraries and their application in studying several important biological problems are also reported.
摘要
肽库是研究蛋白质结构与功能关系的有用工具。创建具有游离C末端的肽库面临独特的合成挑战。在这篇综述中，描述了使用固相肽合成或噬菌体展示创建肽库的方法。还报道了筛选此类文库的方法及其在研究几个重要生物学问题中的应用。
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