在注射了大鼠小肠mRNA的非洲爪蟾卵母细胞中功能性D-葡萄糖转运系统的表达。

Expression of the functional D-glucose transport system in Xenopus oocytes injected with mRNA of rat small intestine.

作者信息

Aoshima H, Ishii H, Anan M

机构信息

Department of Chemistry, Faculty of Liberal Arts, Yamaguchi University, Japan.

出版信息

Brain Res. 1987 Sep;388(3):263-7. doi: 10.1016/0169-328x(87)90033-7.

DOI:10.1016/0169-328x(87)90033-7

PMID:2823960

Abstract

mRNA prepared from rat small intestine was injected into Xenopus oocytes. The injected oocytes showed a clear electrical response to D-glucose (Glu) in the form of membrane depolarization and conductance increase, while none was shown to D-fructose. The membrane electrical response of the injected oocytes evoked by Glu followed the Michaelis-Menten type kinetics and was dependent on the membrane potential of the oocyte. Replacing the Na+ of the bathing buffer with choline+ resulted in no response to Glu. Thus, a Glu transport system coupled to a Na+ gradient was expressed in Xenopus oocytes by injecting mRNA from rat small intestine.

摘要

将从大鼠小肠制备的信使核糖核酸（mRNA）注射到非洲爪蟾卵母细胞中。注射后的卵母细胞对D-葡萄糖（Glu）表现出明显的电反应，形式为膜去极化和电导增加，而对D-果糖则无反应。Glu诱发的注射后卵母细胞的膜电反应遵循米氏动力学类型，并且依赖于卵母细胞的膜电位。用胆碱离子（choline+）取代浴液缓冲液中的钠离子（Na+）导致对Glu无反应。因此，通过注射来自大鼠小肠的mRNA，在非洲爪蟾卵母细胞中表达了一种与Na+梯度偶联的Glu转运系统。