Shepherd E A, Garza J J, Greiner S P, Bowdridge S A
Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, USA.
Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
Parasite Immunol. 2017 Apr;39(4). doi: 10.1111/pim.12424. Epub 2017 Apr 6.
The objective of this study was to determine the effects of peripheral blood mononuclear cells (PBMC), derived from parasite-resistant St. Croix (STC) hair sheep and parasite-susceptible Suffolk (SUF) sheep, on Haemonchus contortus L stage larval death in vitro, with or without autologous serum. Larval morbidity was quantified by measuring larval ATP concentration following incubation with PBMC. Larvae exposed to either STC- or SUF-derived PBMC had lower ATP than live larvae (0.12 μmol/L ATP and 0.16 μmol/L ATP vs 0.27 μmol/L ATP, respectively) (P<.001) and greater ATP of dead larvae (0.03 μmol/L ATP) (P<.001). Breed differences were observed with addition of autologous serum. Larvae exposed to SUF-derived PBMC with autologous serum were not significantly different from live larval ATP. STC-derived serum did not significantly reduce larval ATP compared to PBMC alone (0.11 μmol/L ATP), but was significantly reduced compared to live larvae (0.22 μmol/L ATP) and SUF-derived PBMC with autologous serum (0.23 μmol/L ATP) (P<.001). These data indicate that a cellular response alone is capable of significantly reducing larval ATP in a breed-independent manner. However, addition of serum to SUF-PBMC failed to reduce larval ATP, indicating breed-dependent humoral response to H. contortus.
本研究的目的是确定源自抗寄生虫的圣克罗伊（STC）毛羊和易感寄生虫的萨福克（SUF）羊的外周血单个核细胞（PBMC）在有或无自体血清的情况下，对体外捻转血矛线虫L期幼虫死亡的影响。通过测量与PBMC孵育后幼虫的ATP浓度来量化幼虫的发病率。暴露于源自STC或SUF的PBMC的幼虫的ATP低于活幼虫（分别为0.12μmol/L ATP和0.16μmol/L ATP，而活幼虫为0.27μmol/L ATP）（P<0.001），且死亡幼虫的ATP更高（0.03μmol/L ATP）（P<0.001）。添加自体血清后观察到品种差异。暴露于含有自体血清的源自SUF的PBMC的幼虫与活幼虫的ATP没有显著差异。与单独的PBMC相比（0.11μmol/L ATP），源自STC的血清并没有显著降低幼虫的ATP，但与活幼虫（0.22μmol/L ATP）和含有自体血清的源自SUF的PBMC相比（0.23μmol/L ATP）显著降低（P<0.001）。这些数据表明，仅细胞反应就能以品种无关的方式显著降低幼虫的ATP。然而，向SUF-PBMC中添加血清未能降低幼虫的ATP，表明对捻转血矛线虫存在品种依赖性的体液反应。
