Miotto Benoit
Inserm U1016, Institut Cochin, Paris, France; CNRS, UMR8104, Paris, France - Université Paris Descartes, Sorbonne Paris Cité, 24, rue du Faubourg Saint-Jacques, 75014 Paris, France.
Med Sci (Paris). 2017 Feb;33(2):143-150. doi: 10.1051/medsci/20173302009. Epub 2017 Feb 27.
Eukaryotic DNA replication starts at multiple sites distributed throughout the genome called origins of replication. The identification of the position of these origins in the genome, as well as the description of their sequence features and chromatin structure, have been the center of many studies over the last years. A handful of sophisticated methods has been developed to capture replication initiation events, to detect replication factors binding sites onto the chromatin and to computationally analyze these data at the genome scale. Yet, we remain far from fully understanding how these origins of replication are specified in the genome and how DNA replication initiation is regulated. The aim of this review is to provide the reader with a detailed and curated list of the latest findings regarding the nature of origins of replication in the human genome.
真核生物的DNA复制起始于基因组中分布在各处的多个位点，这些位点被称为复制起点。在过去几年里，确定这些起点在基因组中的位置，以及描述它们的序列特征和染色质结构，一直是众多研究的核心。人们已经开发出了一些复杂的方法来捕捉复制起始事件，检测复制因子在染色质上的结合位点，并在基因组尺度上对这些数据进行计算分析。然而，我们距离完全理解这些复制起点在基因组中是如何被确定的，以及DNA复制起始是如何被调控的，仍有很大差距。这篇综述的目的是为读者提供一份详细且经过精心整理的清单，列出有关人类基因组中复制起点性质的最新研究发现。
