胰岛素结合的物种特异性与胰岛素受体蛋白酪氨酸激酶活性

Species specificity of insulin binding and insulin receptor protein tyrosine kinase activity.

作者信息

Cordera R, Andraghetti G, Gherzi R, Adezati L, Montemurro A, Lauro R, Goldfine I D, De Pirro R

机构信息

Istituto Scientifico di Medicina Interna, Universita' di Genova, Italy.

出版信息

Endocrinology. 1987 Dec;121(6):2007-10. doi: 10.1210/endo-121-6-2007.

DOI:10.1210/endo-121-6-2007

PMID:2824177

Abstract

The effect of monoclonal anti-insulin receptor antibody MA 10 on [125I]insulin binding and on insulin receptor protein tyrosine kinase activity was investigated in human and rat tissues. It was observed that MA 10 inhibits insulin binding to human, but not rat, tissues while inhibiting insulin-stimulated receptor autophosphorylation and protein tyrosine kinase activity in both human and rat tissues. These data suggest that MA 10 is directed against a region of the insulin receptor that is in between the insulin-binding domain and the beta-subunit and that in human, but not rat, tissues, this region is involved in insulin binding.

摘要

在人和大鼠组织中研究了单克隆抗胰岛素受体抗体MA 10对[125I]胰岛素结合以及胰岛素受体蛋白酪氨酸激酶活性的影响。观察到MA 10抑制胰岛素与人组织而非大鼠组织的结合，同时抑制人和大鼠组织中胰岛素刺激的受体自身磷酸化和蛋白酪氨酸激酶活性。这些数据表明，MA 10针对的是胰岛素受体位于胰岛素结合结构域和β亚基之间的一个区域，并且在人组织而非大鼠组织中，该区域参与胰岛素结合。