Mariella J, Castagnetti C, Prosperi A, Scagliarini A, Peli A
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy.
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy.
Vet Immunol Immunopathol. 2017 Mar;185:34-37. doi: 10.1016/j.vetimm.2017.01.007. Epub 2017 Jan 27.
The purpose of this study is to investigate the presence of IL-4, IL-8, IL-13 and IFN-γ in equine colostrum and in foals' serum. Samples were obtained from 14 mares and their healthy foals. Soon after parturition, 10ml of colostrum was collected, filtered, centrifuged and frozen until assayed. Blood samples were obtained from each foal at birth (TO) and again after 24h (T24), after which they were frozen until assayed. Serum IgG was measured at 24h of age with an immunoturbidimetric quantitative method. Cytokine concentration was determined using commercially available ELISA tests. Statistical analyses revealed a significant difference in serum concentration of IL-4 at T0 and at T24 (p<0.05) and a significant correlation between the serum IL-4 at T24 and colostral IL-4. These results suggest the absorption of IL-4 from colostrum. The presence of IL-8 in the pre-suckle foal's serum may be due to an endogenous production. With the exception of two samples, there was no IL-13 detected in the foals' serum at birth and remained undetectable in 8/14 samples after 24h. This cytokine was also undetectable in four colostrum samples, where its concentration showed a wide range and a high standard deviation. IFN-γ was present in both the colostrum and in the foals serum at birth.
本研究的目的是调查白细胞介素-4（IL-4）、白细胞介素-8（IL-8）、白细胞介素-13（IL-13）和干扰素-γ（IFN-γ）在马初乳和驹血清中的存在情况。样本取自14匹母马及其健康的驹。分娩后不久，采集10毫升初乳，过滤、离心并冷冻，直至进行检测。在每匹驹出生时（T0）和24小时后（T24）采集血样，然后冷冻直至检测。在驹24小时龄时，采用免疫比浊定量法测量血清免疫球蛋白G（IgG）。使用市售的酶联免疫吸附测定（ELISA）试验测定细胞因子浓度。统计分析显示，T0和T24时血清IL-4浓度存在显著差异（p<0.05），且T24时血清IL-4与初乳IL-4之间存在显著相关性。这些结果表明初乳中的IL-4被吸收。吮乳前驹血清中IL-8的存在可能是由于内源性产生。除两份样本外，驹出生时血清中未检测到IL-13，24小时后14份样本中有8份仍未检测到。在四份初乳样本中也未检测到这种细胞因子，其浓度显示出广泛的范围和高标准差。出生时初乳和驹血清中均存在IFN-γ。
